Designing functionally versatile, highly immunogenic peptide-based multiepitopic vaccines against foot-and-mouth disease virus

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• dc.contributor.author Defaus, Sira
• dc.contributor.author Forner, Mar, 1980-
• dc.contributor.author Cañas Arranz, Rodrigo
• dc.contributor.author de León, Patricia
• dc.contributor.author Bustos, María J.
• dc.contributor.author Rodríguez Pulido, Miguel
• dc.contributor.author Blanco, Esther
• dc.contributor.author Sobrino, Francisco
• dc.contributor.author Andreu Martínez, David
• dc.date.accessioned 2020-09-08T06:55:49Z
• dc.date.available 2020-09-08T06:55:49Z
• dc.date.issued 2020
• dc.description.abstract A broadly protective and biosafe vaccine against foot-and-mouth disease virus (FMDV) remains an unmet need in the animal health sector. We have previously reported solid protection against serotype O FMDV afforded by dendrimeric peptide structures harboring virus-specific B- and T-cell epitopes, and also shown such type of multivalent presentations to be advantageous over simple B-T-epitope linear juxtaposition. Chemically, our vaccine platforms are modular constructions readily made from specified B- and T-cell epitope precursor peptides that are conjugated in solution. With the aim of developing an improved version of our formulations to be used for on-demand vaccine applications, we evaluate in this study a novel design for epitope presentation to the immune system based on a multiple antigen peptide (MAP) containing six immunologically relevant motifs arranged in dendrimeric fashion (named B2T-TB2). Interestingly, two B2T units fused tail-to-tail into a single homodimer platform elicited higher B- and T-cell specific responses than former candidates, with immunization scores remaining stable even after 4 months. Moreover, this macromolecular assembly shows consistent immune response in swine, the natural FMDV host, at reduced dose. Thus, our versatile, immunogenic prototype can find application in the development of peptide-based vaccine candidates for various therapeutic uses using safer and more efficacious vaccination regimens.
• dc.description.sponsorship This research was funded by Spanish Ministry of Science, Innovation and Universities grants AGL2014-48923-C2 and AGL2017-84097-C2-2-R (to D.A. and F.S.), and AGL2016-349 76445-R to E.B., as well as by Comunidad de Madrid co-financed ECFEDER funds (S2013/ABI-350 2906-PLATESA and P2018/BAA-4370 to F.S. and E.B.), and by Generalitat de Catalunya (2009SGR492 to D.A.).
• dc.format.mimetype application/pdf
• dc.identifier.citation Defaus S, Forner M, Cañas-Arranz R, de León P, Bustos MJ, Rodríguez-Pulido M, Blanco E, Sobrino F, Andreu D. Designing functionally versatile, highly immunogenic peptide-based multiepitopic vaccines against foot-and-mouth disease virus. Vaccines (Basel). 2020; 8(3):E406. DOI: 10.3390/vaccines8030406
• dc.identifier.doi http://dx.doi.org/10.3390/vaccines8030406
• dc.identifier.issn 2076-393X
• dc.identifier.uri http://hdl.handle.net/10230/45266
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Vaccines (Basel). 2020; 8(3):E406
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/AGL2014-48923-C2
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/AGL2017-84097-C2-2-R
• dc.rights © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Click chemistry
• dc.subject.keyword Foot-and-mouth disease virus
• dc.subject.keyword Multivalency
• dc.subject.keyword Peptide-based vaccines
• dc.subject.keyword Swine host
• dc.title Designing functionally versatile, highly immunogenic peptide-based multiepitopic vaccines against foot-and-mouth disease virus
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion