Citizen-science based study of the oral microbiome in Cystic fibrosis and matched controls reveals major differences in diversity and abundance of bacterial and fungal species

Willis, Jesse R.; Saus Martínez, Ester; Iraola Guzmán, Susana; Cabello-Yeves, Elena; Ksiezopolska, Ewa; Cozzuto, Luca; Bejarano, Luis A.; Andreu Somavilla, Núria; Alloza-Trabado, Miriam; Blanco, Andrea; Puig-Sola, Anna; Broglio, Elisabetta; Carolis, Carlo; Ponomarenko, Julia; Hecht, Jochen; Gabaldón Estevan, Juan Antonio, 1973-

Citizen-science based study of the oral microbiome in Cystic fibrosis and matched controls reveals major differences in diversity and abundance of bacterial and fungal species

Mostra el registre complet Registre parcial de l'ítem

dc.contributor.author Willis, Jesse R.
dc.contributor.author Saus Martínez, Ester
dc.contributor.author Iraola Guzmán, Susana
dc.contributor.author Cabello-Yeves, Elena
dc.contributor.author Ksiezopolska, Ewa
dc.contributor.author Cozzuto, Luca
dc.contributor.author Bejarano, Luis A.
dc.contributor.author Andreu Somavilla, Núria
dc.contributor.author Alloza-Trabado, Miriam
dc.contributor.author Blanco, Andrea
dc.contributor.author Puig-Sola, Anna
dc.contributor.author Broglio, Elisabetta
dc.contributor.author Carolis, Carlo
dc.contributor.author Ponomarenko, Julia
dc.contributor.author Hecht, Jochen
dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-
dc.date.accessioned 2021-11-26T06:48:49Z
dc.date.available 2021-11-26T06:48:49Z
dc.date.issued 2021
dc.description.abstract Introduction: Cystic fibrosis (CF) is an autosomal genetic disease, associated with the production of excessively thick mucosa and with life-threatening chronic lung infections. The microbiota of the oral cavity can act as a reservoir or as a barrier for infectious microorganisms that can colonize the lungs. However, the specific composition of the oral microbiome in CF is poorly understood.Methods: In collaboration with CF associations in Spain, we collected oral rinse samples from 31 CF persons (age range 7-47) and matched controls, and then performed 16S rRNA metabarcoding and high-throughput sequencing, combined with culture and proteomics-based identification of fungi to survey the bacterial and fungal oral microbiome.Results: We found that CF is associated with less diverse oral microbiomes, which were characterized by higher prevalence of Candida albicans and differential abundances of a number of bacterial taxa that have implications in both the connection to lung infections in CF, as well as potential oral health concerns, particularly periodontitis and dental caries.Conclusion: Overall, our study provides a first global snapshot of the oral microbiome in CF. Future studies are required to establish the relationships between the composition of the oral and lung microbiomes in CF.
dc.description.sponsorship The project was financed by the CRG through Genomics and Bioinformatics Core Facilities funds, and by the EduCaixa programme through funds from the Fundación Bancaria ‘La Caixa’, with the participation of the Center for Research into Environmental Epidemiology (CREAL), and the ‘Center d’Excellència Severo Ochoa 2013-2017’ programme (SEV-2012-02-08) of the Ministry of Economy and Competitiveness. Eppendorf, Illumina, and ThermoFisher sponsored the research by donating some materials and reagents. TG group acknowledges support from the Spanish Ministry of Science and Innovation for grant PGC2018-099921-B-I00, cofounded by European Regional Development Fund (ERDF); from the CERCA Programme/Generalitat de Catalunya; from the Catalan Research Agency (AGAUR) SGR423; from the European Union’s Horizon 2020 research and innovation programme under the grant agreement ERC-2016-724173; from Instituto de Salud Carlos III (INB Grant PT17/0009/0023 - ISCIII-SGEFI/ERDF).
dc.format.mimetype application/pdf
dc.identifier.citation Willis JR, Saus E, Iraola-Guzmán S, Cabello-Yeves E, Ksiezopolska E, Cozzuto L, Bejarano LA, Andreu-Somavilla N, Alloza-Trabado M, Blanco A, Puig-Sola A, Broglio E, Carolis C, Ponomarenko J, Hecht J, Gabaldón T. Citizen-science based study of the oral microbiome in Cystic fibrosis and matched controls reveals major differences in diversity and abundance of bacterial and fungal species. J Oral Microbiol. 2021;13(1):1897328. DOI: 10.1080/20002297.2021.1897328
dc.identifier.doi http://dx.doi.org/10.1080/20002297.2021.1897328
dc.identifier.issn 2000-2297
dc.identifier.uri http://hdl.handle.net/10230/49063
dc.language.iso eng
dc.publisher Taylor & Francis
dc.relation.ispartof J Oral Microbiol. 2021;13(1):1897328
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/724173
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-099921-B-I00
dc.rights © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
dc.subject.keyword Candida
dc.subject.keyword Cystic fibrosis
dc.subject.keyword Oral microbiome
dc.subject.keyword Pseudomonas
dc.subject.keyword Oral mycobiome
dc.title Citizen-science based study of the oral microbiome in Cystic fibrosis and matched controls reveals major differences in diversity and abundance of bacterial and fungal species
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

Col·leccions

Articles (Center for Genomic Regulation (CRG))
Articles (Departament de Medicina i Ciències de la Vida)
Documents OpenAIRE (Open Access Infrastructure for Research in Europe)