Citizen-science based study of the oral microbiome in Cystic fibrosis and matched controls reveals major differences in diversity and abundance of bacterial and fungal species
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- dc.contributor.author Willis, Jesse R.
- dc.contributor.author Saus Martínez, Ester
- dc.contributor.author Iraola Guzmán, Susana
- dc.contributor.author Cabello-Yeves, Elena
- dc.contributor.author Ksiezopolska, Ewa
- dc.contributor.author Cozzuto, Luca
- dc.contributor.author Bejarano, Luis A.
- dc.contributor.author Andreu Somavilla, Núria
- dc.contributor.author Alloza-Trabado, Miriam
- dc.contributor.author Blanco, Andrea
- dc.contributor.author Puig-Sola, Anna
- dc.contributor.author Broglio, Elisabetta
- dc.contributor.author Carolis, Carlo
- dc.contributor.author Ponomarenko, Julia
- dc.contributor.author Hecht, Jochen
- dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-
- dc.date.accessioned 2021-11-26T06:48:49Z
- dc.date.available 2021-11-26T06:48:49Z
- dc.date.issued 2021
- dc.description.abstract Introduction: Cystic fibrosis (CF) is an autosomal genetic disease, associated with the production of excessively thick mucosa and with life-threatening chronic lung infections. The microbiota of the oral cavity can act as a reservoir or as a barrier for infectious microorganisms that can colonize the lungs. However, the specific composition of the oral microbiome in CF is poorly understood.Methods: In collaboration with CF associations in Spain, we collected oral rinse samples from 31 CF persons (age range 7-47) and matched controls, and then performed 16S rRNA metabarcoding and high-throughput sequencing, combined with culture and proteomics-based identification of fungi to survey the bacterial and fungal oral microbiome.Results: We found that CF is associated with less diverse oral microbiomes, which were characterized by higher prevalence of Candida albicans and differential abundances of a number of bacterial taxa that have implications in both the connection to lung infections in CF, as well as potential oral health concerns, particularly periodontitis and dental caries.Conclusion: Overall, our study provides a first global snapshot of the oral microbiome in CF. Future studies are required to establish the relationships between the composition of the oral and lung microbiomes in CF.
- dc.description.sponsorship The project was financed by the CRG through Genomics and Bioinformatics Core Facilities funds, and by the EduCaixa programme through funds from the Fundación Bancaria ‘La Caixa’, with the participation of the Center for Research into Environmental Epidemiology (CREAL), and the ‘Center d’Excellència Severo Ochoa 2013-2017’ programme (SEV-2012-02-08) of the Ministry of Economy and Competitiveness. Eppendorf, Illumina, and ThermoFisher sponsored the research by donating some materials and reagents. TG group acknowledges support from the Spanish Ministry of Science and Innovation for grant PGC2018-099921-B-I00, cofounded by European Regional Development Fund (ERDF); from the CERCA Programme/Generalitat de Catalunya; from the Catalan Research Agency (AGAUR) SGR423; from the European Union’s Horizon 2020 research and innovation programme under the grant agreement ERC-2016-724173; from Instituto de Salud Carlos III (INB Grant PT17/0009/0023 - ISCIII-SGEFI/ERDF).
- dc.format.mimetype application/pdf
- dc.identifier.citation Willis JR, Saus E, Iraola-Guzmán S, Cabello-Yeves E, Ksiezopolska E, Cozzuto L, Bejarano LA, Andreu-Somavilla N, Alloza-Trabado M, Blanco A, Puig-Sola A, Broglio E, Carolis C, Ponomarenko J, Hecht J, Gabaldón T. Citizen-science based study of the oral microbiome in Cystic fibrosis and matched controls reveals major differences in diversity and abundance of bacterial and fungal species. J Oral Microbiol. 2021;13(1):1897328. DOI: 10.1080/20002297.2021.1897328
- dc.identifier.doi http://dx.doi.org/10.1080/20002297.2021.1897328
- dc.identifier.issn 2000-2297
- dc.identifier.uri http://hdl.handle.net/10230/49063
- dc.language.iso eng
- dc.publisher Taylor & Francis
- dc.relation.ispartof J Oral Microbiol. 2021;13(1):1897328
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/724173
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-099921-B-I00
- dc.rights © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
- dc.subject.keyword Candida
- dc.subject.keyword Cystic fibrosis
- dc.subject.keyword Oral microbiome
- dc.subject.keyword Pseudomonas
- dc.subject.keyword Oral mycobiome
- dc.title Citizen-science based study of the oral microbiome in Cystic fibrosis and matched controls reveals major differences in diversity and abundance of bacterial and fungal species
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion