A unique cluster of roo insertions in the promoter region of a stress response gene in Drosophila melanogaster

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• dc.contributor.author Merenciano, Miriam
• dc.contributor.author Iacometti, Camillo
• dc.contributor.author González, Josefa
• dc.date.accessioned 2020-07-09T06:46:08Z
• dc.date.available 2020-07-09T06:46:08Z
• dc.date.issued 2019
• dc.description.abstract Transposable elements (TEs) are not randomly distributed in the genome. A genome-wide analysis of the D. melanogaster genome found that differences in TE density across 50 kb genomic regions was due both to transposition and duplication. At smaller genomic scales, promoter regions of hsp genes and the promoter region of CG18446 have been shown to accumulate TE insertions. In this work, we have further analyzed the promoter region of CG18446. We screened 218 strains collected in 15 natural populations, and we found that the CG18446 promoter region contains 20 independent roo insertions. Based on phylogenetic analysis, we suggest that the presence of multiple roo insertions in this region is likely to be the result of several bursts of transposition. Moreover, we found that the roo insertional cluster in the CG18446 promoter region is unique: no other promoter region in the genome contains a similar number of roo insertions. We found that, similar to hsp gene promoters, chromatin accessibility could be one of the factors explaining the recurrent insertions of roo elements in CG18446 promoter region.
• dc.description.sponsorship This work was funded by the European Commission (H2020-ERC-2014-CoG-647900). C.I. was funded by an ERASMUS+ fellowship. We acknowledge the support of the Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de laGeneralitat de Catalunya (GRC 2017 SGR 880). We also acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).
• dc.format.mimetype application/pdf
• dc.identifier.citation Merenciano M, Iacometti C, González J. A unique cluster of roo insertions in the promoter region of a stress response gene in Drosophila melanogaster. Mob DNA. 2019; 10:10. DOI: 10.1186/s13100-019-0152-9
• dc.identifier.doi http://dx.doi.org/10.1186/s13100-019-0152-9
• dc.identifier.issn 1759-8753
• dc.identifier.uri http://hdl.handle.net/10230/45093
• dc.language.iso eng
• dc.publisher BioMed Central
• dc.relation.ispartof Mob DNA. 2019; 10:10
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/647900
• dc.rights © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Fecundity
• dc.subject.keyword Natural population
• dc.subject.keyword Recurrent insertion
• dc.subject.keyword Target site duplication
• dc.subject.keyword Transposable element
• dc.subject.keyword Viability
• dc.title A unique cluster of roo insertions in the promoter region of a stress response gene in Drosophila melanogaster
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion