TANGO1 membrane helices create a lipid diffusion barrier at curved membranes
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- dc.contributor.author Raote, Ishier
- dc.contributor.author Ernst, Andreas M.
- dc.contributor.author Campelo, Felix
- dc.contributor.author Rothman, James E.
- dc.contributor.author Pincet, Frederic
- dc.contributor.author Malhotra, Vivek
- dc.date.accessioned 2020-11-18T07:04:52Z
- dc.date.available 2020-11-18T07:04:52Z
- dc.date.issued 2020
- dc.description.abstract We have previously shown TANGO1 organises membranes at the interface of the endoplasmic reticulum (ER) and ERGIC/Golgi (Raote et al., 2018). TANGO1 corrals retrograde membranes at ER exit sites to create an export conduit. Here the retrograde membrane is, in itself, an anterograde carrier. This mode of forward transport necessitates a mechanism to prevent membrane mixing between ER and the retrograde membrane. TANGO1 has an unusual membrane helix organisation, composed of one membrane-spanning helix (TM) and another that penetrates the inner leaflet (IM). We have reconstituted these membrane helices in model membranes and shown that TM and IM together reduce the flow of lipids at a region of defined shape. We have also shown that the helices align TANGO1 around an ER exit site. We suggest this is a mechanism to prevent membrane mixing during TANGO1-mediated transfer of bulky secretory cargos from the ER to the ERGIC/Golgi via a tunnel.
- dc.format.mimetype application/pdf
- dc.identifier.citation Raote I, Ernst AM, Campelo F, Rothman JE, Pincet F, Malhotra V. TANGO1 membrane helices create a lipid diffusion barrier at curved membranes. Elife. 2020; 9:e57822. DOI: 10.7554/eLife.57822
- dc.identifier.doi http://dx.doi.org/10.7554/eLife.57822
- dc.identifier.issn 2050-084X
- dc.identifier.uri http://hdl.handle.net/10230/45795
- dc.language.iso eng
- dc.publisher eLife
- dc.relation.ispartof Elife. 2020; 9:e57822
- dc.rights © 2020, Raote et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Cell biology
- dc.subject.keyword Collagen
- dc.subject.keyword Human
- dc.subject.keyword Intramembrane
- dc.subject.keyword Lipid
- dc.subject.keyword Membrane curvature
- dc.subject.keyword Molecular biophysics
- dc.subject.keyword Secretion
- dc.subject.keyword Structural biology
- dc.subject.keyword Transmembrane
- dc.title TANGO1 membrane helices create a lipid diffusion barrier at curved membranes
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion