Development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with FlagT4G against classical swine fever from infected pigs

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• dc.contributor.author Bohórquez, José Alejandro
• dc.contributor.author Defaus, Sira
• dc.contributor.author Rosell, Rosa
• dc.contributor.author Pérez-Simó, Marta
• dc.contributor.author Alberch, Mònica
• dc.contributor.author Gladue, Douglas P.
• dc.contributor.author Borca, Manuel V.
• dc.contributor.author Andreu Martínez, David
• dc.contributor.author Ganges, Lliliane
• dc.date.accessioned 2021-11-12T07:22:08Z
• dc.date.available 2021-11-12T07:22:08Z
• dc.date.issued 2021
• dc.description.abstract Classical swine fever virus (CSFV) causes a viral disease of high epidemiological and economical significance that affects domestic and wild swine. Control of the disease in endemic countries is based on live-attenuated vaccines (LAVs) that induce an early protective immune response against highly virulent CSFV strains. The main disadvantage of these currently available LAVs is the lack of serological techniques to differentiate between vaccinated and infected animals (DIVA concept). Here, we describe the development of the FlagDIVA test, a serological diagnostic tool allowing for the differentiation between animals vaccinated with the FlagT4G candidate and those infected with CSFV field strains. The FlagDIVA test is a direct ELISA based on a dendrimeric peptide construct displaying a conserved epitope of CSFV structural protein E2. Although FlagDIVA detected anti-CSFV anti-bodies in infected animals, it did not recognize the antibody response of FlagT4G-vaccinated animals. Therefore, the FlagDIVA test constitutes a valuable accessory DIVA tool in implementing vaccination with the FlagT4G candidate.
• dc.format.mimetype application/pdf
• dc.identifier.citation Bohórquez JA, Defaus S, Rosell R, Pérez-Simó M, Alberch M, Gladue DP, Borca MV, Andreu D, Ganges L. Development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with FlagT4G against classical swine fever from infected pigs. Viruses. 2021;13(10):1980. DOI: 10.3390/v13101980
• dc.identifier.doi http://dx.doi.org/10.3390/v13101980
• dc.identifier.issn 1999-4915
• dc.identifier.uri http://hdl.handle.net/10230/48960
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Viruses. 2021;13(10):1980
• dc.rights © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword CSFV live-attenuated vaccine
• dc.subject.keyword FlagT4G CSFV vaccine candidate
• dc.subject.keyword Serological DIVA test
• dc.title Development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with FlagT4G against classical swine fever from infected pigs
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion