A genetic toolkit and gene switches to limit Mycoplasma growth for biosafety applications
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- dc.contributor.author Broto, Alicia
- dc.contributor.author Gaspari, Erika
- dc.contributor.author Miravet Verde, Samuel, 1992-
- dc.contributor.author Martins dos Santos, Vitor
- dc.contributor.author Isalan, Mark
- dc.date.accessioned 2022-05-31T09:59:45Z
- dc.date.available 2022-05-31T09:59:45Z
- dc.date.issued 2022
- dc.description.abstract Mycoplasmas have exceptionally streamlined genomes and are strongly adapted to their many hosts, which provide them with essential nutrients. Owing to their relative genomic simplicity, Mycoplasmas have been used to develop chassis for biotechnological applications. However, the dearth of robust and precise toolkits for genomic manipulation and tight regulation has hindered any substantial advance. Herein we describe the construction of a robust genetic toolkit for M. pneumoniae, and its successful deployment to engineer synthetic gene switches that control and limit Mycoplasma growth, for biosafety containment applications. We found these synthetic gene circuits to be stable and robust in the long-term, in the context of a minimal cell. With this work, we lay a foundation to develop viable and robust biosafety systems to exploit a synthetic Mycoplasma chassis for live attenuated vectors for therapeutic applications.
- dc.description.sponsorship This work was funded by the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 634942 (MycoSynVac)
- dc.format.mimetype application/pdf
- dc.identifier.citation Broto A, Gaspari E, Miravet-Verde S, Martins Dos Santos VAP, Isalan M. A genetic toolkit and gene switches to limit Mycoplasma growth for biosafety applications. Nat Commun. 2022 Apr 7;13(1):1910. DOI:10.1038/s41467-022-29574-0
- dc.identifier.doi http://dx.doi.org/10.1038/s41467-022-29574-0
- dc.identifier.issn 2041-1723
- dc.identifier.uri http://hdl.handle.net/10230/53326
- dc.language.iso eng
- dc.publisher Nature Research
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/634942
- dc.rights © Alicia Broto et al. 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri https://creativecommons.org/licenses/by/4.0/
- dc.subject.other Genètica
- dc.subject.other Genòmica
- dc.subject.other Micoplasmosis
- dc.title A genetic toolkit and gene switches to limit Mycoplasma growth for biosafety applications
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion