Live-cell structural biology to solve biological mechanisms: the case of the exocyst
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- dc.contributor.author Irastorza-Azcarate, Ibai
- dc.contributor.author Castaño-Díez, Daniel
- dc.contributor.author Devos, Damien P.
- dc.contributor.author Gallego, Oriol
- dc.date.accessioned 2019-10-07T10:50:59Z
- dc.date.issued 2019
- dc.description.abstract Historically, structural biology has been largely centered on in vitro approaches as the dominant technique to obtain indispensable high-resolution data. In situ structural biology is now poised to contribute with high-precision observations in a near-physiological context. Mass spectrometry, electron tomography, and fluorescence microscopy are opening up new opportunities for structural analysis, including the study of the protein machinery in living cells. The complementarity between studies is increasingly used to reveal biologically significant observations. Here we compare two complementary studies addressing the mechanisms of vesicle tethering with in vitro and in situ approaches. Cryoelectron microscopy and live-cell imaging assisted by anchoring platforms team up to explore elusive mechanisms of exocytosis, showing directions of future research.
- dc.format.mimetype application/pdf
- dc.identifier.citation Irastorza-Azcarate I, Castaño-Díez D, Devos DP, Gallego O. Live-cell structural biology to solve biological mechanisms: the case of the exocyst. Structure. 2019;27(6):886-92. DOI: 10.1016/j.str.2019.04.010
- dc.identifier.doi http://dx.doi.org/10.1016/j.str.2019.04.010
- dc.identifier.issn 0969-2126
- dc.identifier.uri http://hdl.handle.net/10230/42399
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof Structure. 2019;27(6):886-92
- dc.rights © Elsevier http://dx.doi.org/10.1016/j.str.2019.04.010
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword Cryo-EM
- dc.subject.keyword Cryo-ET
- dc.subject.keyword Exocyst
- dc.subject.keyword Exocytosis
- dc.subject.keyword Integrative structural biology
- dc.subject.keyword Light microscopy
- dc.subject.keyword Molecular mechanisms in membrane biology
- dc.title Live-cell structural biology to solve biological mechanisms: the case of the exocyst
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/acceptedVersion