Nucleosome-driven transcription factor binding and gene regulation

Ballaré, Cecilia Julia; Castellano, Giancarlo; Gaveglia, Laura; Althammer, Sonja Daniela; González-Vallinas Rostes, Juan, 1983-; Eyras Jiménez, Eduardo; Le Dily, François; Zaurín Quer, Roser; Soronellas, Daniel; Vicent, Guillermo Pablo; Beato, Miguel

Nucleosome-driven transcription factor binding and gene regulation

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dc.contributor.author Ballaré, Cecilia Juliaca
dc.contributor.author Castellano, Giancarloca
dc.contributor.author Gaveglia, Lauraca
dc.contributor.author Althammer, Sonja Danielaca
dc.contributor.author González-Vallinas Rostes, Juan, 1983-ca
dc.contributor.author Eyras Jiménez, Eduardoca
dc.contributor.author Le Dily, Françoisca
dc.contributor.author Zaurín Quer, Roserca
dc.contributor.author Soronellas, Danielca
dc.contributor.author Vicent, Guillermo Pabloca
dc.contributor.author Beato, Miguelca
dc.date.accessioned 2015-12-01T15:06:12Z
dc.date.available 2015-12-01T15:06:12Z
dc.date.issued 2013
dc.description.abstract In fission yeast cells, Cds1 is the effector kinase of the DNA replication checkpoint. We previously showed that when the DNA replication checkpoint is activated, the repressor Yox1 is phosphorylated and inactivated by Cds1, resulting in activation of MluI-binding factor (MBF)-dependent transcription. This is essential to reinitiate DNA synthesis and for correct G1-to-S transition. Here we show that Cdc10, which is an essential part of the MBF core, is the target of the DNA damage checkpoint. When fission yeast cells are treated with DNA-damaging agents, Chk1 is activated and phosphorylates Cdc10 at its carboxy-terminal domain. This modification is responsible for the repression of MBF-dependent transcription through induced release of MBF from chromatin. This inactivation of MBF is important for survival of cells challenged with DNA-damaging agents. Thus Yox1 and Cdc10 couple normal cell cycle regulation in unperturbed conditions and the DNA replication and DNA damage checkpoints into a single transcriptional complex.ca
dc.description.sponsorship The experimental work was supported by grants from the Spanish government (BMC 2003-02902 and 2010-15313; CSD2006-00049), the European Union (IP HEROIC), and the Catalan government (AGAUR). L.G. was a recipient of a fellowship from the International PhD program of LaCaixa; G.P.V. was a recipient of a fellowship from the Ramón y Cajal program.
dc.format.mimetype application/pdfca
dc.identifier.citation Ballaré C, Castellano G, Gaveglia L, Althammer S, González-Vallinas J, Eyras E et al. Nucleosome-driven transcription factor binding and gene regulation. Molecular cell. 2013;49(1):67-79. DOI: 10.1016/j.molcel.2012.10.019ca
dc.identifier.doi http://dx.doi.org/10.1016/j.molcel.2012.10.019
dc.identifier.issn 1097-2765
dc.identifier.uri http://hdl.handle.net/10230/25285
dc.language.iso engca
dc.publisher Elsevierca
dc.relation.ispartof Molecular cell. 2013;49(1):67-79
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PN/BMC2003-02902
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BMC2010-15313
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/CSD2006-00049
dc.rights © Elsevier. This is the published version of an article http://10.1016/j.molcel.2012.10.019 that appeared in the journal Molecular cell. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-licenseen
dc.rights.accessRights info:eu-repo/semantics/openAccessca
dc.subject.other Gens humans Mapatgeca
dc.subject.other Cromatinaca
dc.title Nucleosome-driven transcription factor binding and gene regulationca
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/publishedVersionca

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