Chromogenic LMO2 mRNA ISH expression correlates with LMO2 protein and gene expression and captures their survival impact in diffuse large B-Cell Lymphoma, NOS
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- dc.contributor.author Papaleo, Natalia
- dc.contributor.author Molina-Alvarez, Andrea
- dc.contributor.author Onieva, Ricard
- dc.contributor.author Fuertes, Diana
- dc.contributor.author Sanchez-Gonzalez, Blanca
- dc.contributor.author Riera, Xènia
- dc.contributor.author Lopez-Segura, David
- dc.contributor.author Lome-Maldonado, Carmen
- dc.contributor.author Ara-Mancebo, Xavier
- dc.contributor.author Yélamos López, José
- dc.contributor.author Salido Galeote, Marta
- dc.contributor.author Vázquez, Ivonne
- dc.contributor.author Calvo, Xavier
- dc.contributor.author Colomo Saperas, Luis Alberto
- dc.date.accessioned 2025-03-10T06:57:20Z
- dc.date.available 2025-03-10T06:57:20Z
- dc.date.issued 2024
- dc.description.abstract Background: LMO2 is a relevant gene involved in B-cell ontogeny and a survival predictor of aggressive large B-cell lymphomas (aLBCL). Most studies assessing LMO2 mRNA expression have relied on microarray platforms or qRT-PCR methods, overlooking tissue morphology. In this study, we evaluate LMO2 RNA expression by chromogenic in situ hybridization (CISH) in normal tissue and in a series of 82 aLBCL. Methods: LMO2 CISH was performed in formalin-fixed paraffin-embedded tissues, scored by three different methods, and correlated with a transcriptome panel. Results: We obtained statistically significant results correlating the methods of evaluation with LMO2 protein expression and gene expression results. Normal tonsil tissue showed high levels of LMO2, particularly within the light zone of the germinal center. Conversely, in aLBCL, a notable reduction in LMO2 expression was noted, remarkably in cases carrying MYC rearrangements. Furthermore, significant results were obtained through overall survival and Cox regression survival analysis, incorporating International Prognostic Index data alongside LMO2 expression levels. Conclusions: We show a reliable method to identify LMO2 mRNA expression by CISH, effectively capturing many of the reported biologic features of LMO2.
- dc.format.mimetype application/pdf
- dc.identifier.citation Papaleo N, Molina-Alvarez A, Onieva R, Fuertes D, Sanchez-Gonzalez B, Riera X, et al. Chromogenic LMO2 mRNA ISH expression correlates with LMO2 protein and gene expression and captures their survival impact in diffuse large B-Cell Lymphoma, NOS. Cancers (Basel). 2024 Jun 28;16(13):2378. DOI: 10.3390/cancers16132378
- dc.identifier.doi http://dx.doi.org/10.3390/cancers16132378
- dc.identifier.issn 2072-6694
- dc.identifier.uri http://hdl.handle.net/10230/69888
- dc.language.iso eng
- dc.publisher MDPI
- dc.relation.ispartof Cancers (Basel). 2024 Jun 28;16(13):2378
- dc.rights © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword CISH
- dc.subject.keyword LMO2
- dc.subject.keyword MYC
- dc.subject.keyword Large B-cell lymphoma
- dc.title Chromogenic LMO2 mRNA ISH expression correlates with LMO2 protein and gene expression and captures their survival impact in diffuse large B-Cell Lymphoma, NOS
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion