Multicenter experiment for quality control of peptide-centric LC-MS/MS analysis - A longitudinal performance assessment with nLC coupled to orbitrap MS analyzers

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• dc.contributor.author Campos, Alexca
• dc.contributor.author Díaz, Ramónca
• dc.contributor.author Martínez Bartolomé, Salvadorca
• dc.contributor.author Sierra, Joseca
• dc.contributor.author Gallardo, Oscarca
• dc.contributor.author Sabidó Aguadé, Eduard, 1981-ca
• dc.contributor.author López Lucendo, Maríaca
• dc.contributor.author Casal, José Ignacioca
• dc.contributor.author Pasquarello, Carlaca
• dc.contributor.author Scherl, Alexanderca
• dc.contributor.author Chiva, Cristinaca
• dc.contributor.author Borrás, Evaca
• dc.contributor.author Odena, Antoniaca
• dc.contributor.author Elortza, Félixca
• dc.contributor.author Azkargorta, Mikelca
• dc.contributor.author Ibarrola, Nievesca
• dc.contributor.author Canals, Francescca
• dc.contributor.author Albar, Juan Pabloca
• dc.contributor.author Oliveira, Eliandreca
• dc.date.accessioned 2016-07-25T12:31:34Z
• dc.date.available 2016-09-08T02:00:03Z
• dc.date.issued 2015
• dc.description.abstract Proteomic technologies based on mass spectrometry (MS) have greatly evolved in the past years, and nowadays it is possible to routinely identify thousands of peptides from complex biological samples in a single LC-MS/MS experiment. Despite the advancements in proteomic technologies, the scientific community still faces important challenges in terms of depth and reproducibility of proteomics analyses. Here, we present a multicenter study designed to evaluate long-term performance of LC-MS/MS platforms within the Spanish Proteomics Facilities Network (ProteoRed-ISCIII). The study was performed under well-established standard operating procedures, and demonstrated that it is possible to attain qualitative and quantitative reproducibility over time. Our study highlights the importance of deploying quality assessment metrics routinely in individual laboratories and in multi-laboratory studies. The mass spectrometry data have been deposited to the ProteomeXchange Consortium with the data set identifier PXD000205.This article is part of a Special Issue entitled: HUPO 2014.ca
• dc.description.sponsorship This work was supported by the Instituto de Salud Carlos III (ProteoRed grant #2005X747).
• dc.format.mimetype application/pdfca
• dc.identifier.citation Campos A, Díaz R, Martínez-Bartolomé S, Sierra J, Gallardo O, Sabidó E et al. Multicenter experiment for quality control of peptide-centric LC-MS/MS analysis - A longitudinal performance assessment with nLC coupled to orbitrap MS analyzers. Journal of proteomics. 2015;127(Pt B):264-74. DOI: 10.1016/j.jprot.2015.05.012ca
• dc.identifier.doi http://dx.doi.org/10.1016/j.jprot.2015.05.012
• dc.identifier.issn 1874-3919
• dc.identifier.uri http://hdl.handle.net/10230/27116
• dc.language.iso engca
• dc.publisher Elsevierca
• dc.relation.ispartof Journal of proteomics. 2015;127(Pt B):264-74
• dc.rights © Elsevier http://dx.doi.org/10.1016/j.jprot.2015.05.012ca
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.keyword LC-MS/MS performance
• dc.subject.keyword Quality control
• dc.subject.keyword Reproducibility
• dc.subject.keyword Yeast
• dc.subject.other Pèptidsca
• dc.title Multicenter experiment for quality control of peptide-centric LC-MS/MS analysis - A longitudinal performance assessment with nLC coupled to orbitrap MS analyzersca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/acceptedVersionca