A proteomic approach for systematic mapping of substrates of human deubiquitinating enzymes

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• dc.contributor.author Ramirez, Juanma
• dc.contributor.author Prieto, Gorka
• dc.contributor.author Olazabal-Herrero, Anne
• dc.contributor.author Borràs, Eva
• dc.contributor.author Fernandez-Vigo, Elvira
• dc.contributor.author Alduntzin, Unai
• dc.contributor.author Osinalde, Nerea
• dc.contributor.author Beaskoetxea, Javier
• dc.contributor.author Lectez, Benoit
• dc.contributor.author Aloria, Kerman
• dc.contributor.author Rodríguez, José Antonio
• dc.contributor.author Paradela, Alberto
• dc.contributor.author Sabidó Aguadé, Eduard, 1981-
• dc.contributor.author Muñoz, Javier
• dc.contributor.author Corrales, Fernando
• dc.contributor.author Arizmendi, Jesús M.
• dc.contributor.author Mayor, Ugo
• dc.date.accessioned 2021-11-25T06:51:41Z
• dc.date.available 2021-11-25T06:51:41Z
• dc.date.issued 2021
• dc.description.abstract The human genome contains nearly 100 deubiquitinating enzymes (DUBs) responsible for removing ubiquitin moieties from a large variety of substrates. Which DUBs are responsible for targeting which substrates remain mostly unknown. Here we implement the bioUb approach to identify DUB substrates in a systematic manner, combining gene silencing and proteomics analyses. Silencing of individual DUB enzymes is used to reduce their ubiquitin deconjugating activity, leading to an increase of the ubiquitination of their substrates, which can then be isolated and identified. We report here quantitative proteomic data of the putative substrates of 5 human DUBs. Furthermore, we have built a novel interactive database of DUB substrates to provide easy access to our data and collect DUB proteome data from other groups as a reference resource in the DUB substrates research field.
• dc.description.sponsorship Authors of this paper are members of Proteored, PRB3 and are supported by grant PT17/0019 of the PE I+D+i 2013-2016, funded by Instituto de Salud Carlos III (ISCIII) and European Regional Development Fund (ERDF). This research was also funded by the Spanish Ministry of Economy, grant number SAF2016-76898-P and the University of the Basque Country (UPV/EHU), grant number US19/05. J.R was supported with a postdoctoral research fellowship from the UPV/EHU.
• dc.format.mimetype application/pdf
• dc.identifier.citation Ramirez J, Prieto G, Olazabal-Herrero A, Borràs E, Fernandez-Vigo E, Alduntzin U, Osinalde N, Beaskoetxea J, Lectez B, Aloria K, Rodriguez JA, Paradela A, Sabidó E, Muñoz J, Corrales F, Arizmendi JM, Mayor U. A proteomic approach for systematic mapping of substrates of human deubiquitinating enzymes. Int J Mol Sci. 2021;22(9):4851. DOI: 10.3390/ijms22094851
• dc.identifier.doi http://dx.doi.org/10.3390/ijms22094851
• dc.identifier.issn 1422-0067
• dc.identifier.uri http://hdl.handle.net/10230/49053
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Int J Mol Sci. 2021;22(9):4851
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2016-76898-P
• dc.rights © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword DUBase
• dc.subject.keyword Deubiquitinating enzyme
• dc.subject.keyword Quantitative proteomics
• dc.subject.keyword Ubiquitination
• dc.title A proteomic approach for systematic mapping of substrates of human deubiquitinating enzymes
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion