Prenatal alcohol exposure dysregulates the expression of clock genes and alters rhythmic behaviour in mice

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• dc.contributor.author Reina-Campos, Maria
• dc.contributor.author Gallego-Landin, Ines
• dc.contributor.author Medrano, Mireia
• dc.contributor.author García Baos, Alba
• dc.contributor.author Valverde Granados, Olga
• dc.date.accessioned 2025-11-18T17:03:08Z
• dc.date.available 2025-11-18T17:03:08Z
• dc.date.issued 2025
• dc.date.updated 2025-11-18T17:03:08Z
• dc.description.abstract Foetal alcohol spectrum disorders (FASDs) refer to a range of adverse physical, behavioural and cognitive effects caused by perinatal alcohol exposure. While cognitive impairments are well documented, FASD has also been associated with sleep disturbances and circadian rhythm disruptions. This study aimed to examine the effects of perinatal alcohol exposure on circadian rhythms at behavioural and gene expression levels across two developmental stages (adolescence and adulthood) in both male and female mice. Using a validated prenatal and lactation alcohol exposure (PLAE) protocol, we assessed circadian patterns of locomotor activity under free-running conditions and spatial memory performance during adolescence and adulthood. Additionally, we evaluated the impact of PLAE on circadian expression of clock and non-circadian genes involved in neurotransmission across key brain regions, including the medial prefrontal cortex and hippocampus. PLAE altered circadian rhythmicity and impaired spatial memory. Gene expression analyses revealed disrupted oscillatory patterns in clock genes and in genes related to plasticity and cognition, including those from the expanded endocannabinoid system (e.g. Cnr1, Dagla, Faah) and other neurotransmitter systems (e.g. Oprm1, Slc17a8, Drd1, Gabra1). These findings underscore the impact of early alcohol exposure on biological rhythms and neurobehavioural function, highlighting circadian dysregulation as a contributing factor to FASD.
• dc.description.sponsorship This work was supported by the Ministerio de Ciencia e Innovación, Grant PID2022-136962OB-100-MCIN/AEI/10.13039/501100011033 and by ERDF 'A way of making Europe', Ministerio de Sanidad (Delegación del Gobierno para el Plan Nacional sobre Drogas 2023/005 and Exp2022/008695 Fondos de Recuperación, Transformación y Resiliencia (PRTR) Union Europea), and by the Generalitat de Catalunya, AGAUR (2021SGR00485). O.V. is the recipient of an ICREA Academia Award (Institució Catalana de Recerca i Estudis Avançats, Generalitat de Catalunya).
• dc.format.mimetype application/pdf
• dc.identifier.citation Reina-Campos M, Gallego-Landin I, Medrano M, Garcia-Baos A, Valverde O. Prenatal alcohol exposure dysregulates the expression of clock genes and alters rhythmic behaviour in mice. Open Biology. 2025;15(10):250141. DOI: 10.1098/rsob.250141
• dc.identifier.doi http://dx.doi.org/10.1098/rsob.250141
• dc.identifier.issn 2046-2441
• dc.identifier.uri http://hdl.handle.net/10230/71929
• dc.language.iso eng
• dc.publisher Royal Society
• dc.relation.ispartof Open Biology. 2025;15(10):250141
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2022-136962OB-100
• dc.rights © 2025 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Circadian rhythms
• dc.subject.keyword Drinking in the dark test
• dc.subject.keyword Foetal alcohol spectrum disorders (FASDs)
• dc.subject.keyword Gene expression
• dc.subject.keyword Hippocampal memory
• dc.subject.keyword Locomotor activity
• dc.subject.keyword Mice
• dc.subject.keyword Prenatal and lactation alcohol exposure
• dc.title Prenatal alcohol exposure dysregulates the expression of clock genes and alters rhythmic behaviour in mice
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion