A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave
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- dc.contributor.author Gaspa, Lauraca
- dc.contributor.author González Medina, Alberto, 1990-ca
- dc.contributor.author Hidalgo Hernando, Elenaca
- dc.contributor.author Ayté del Olmo, Joséca
- dc.date.accessioned 2016-04-12T12:30:22Z
- dc.date.issued 2016
- dc.description.abstract The Schizosaccharomyces pombe MBF complex activates the transcription of genes required for DNA synthesis and S phase. The MBF complex contains several proteins, including the core components Cdc10, Res1 and Res2, the co-repressor proteins Yox1 and Nrm1 and the co-activator Rep2. It has recently been shown how MBF is regulated when either the DNA damage or the DNA synthesis checkpoints are activated. However, how MBF is regulated in a normal unperturbed cell cycle is still not well understood. We have set up a genome-wide genomic screen searching for global regulators of MBF. We have crossed our knock-out collection library with a reporter strain that allows the measurement of MBF activity in live cells by flow cytometry. We confirm previously known regulators of MBF and show that COP9/signalosome and tRNA methyltransferases also regulate MBF activityca
- dc.description.sponsorship This work was supported by grants from the Spanish Ministerio de Economia y Competitividad (BFU2012-31939), PLAN E and Feder. E. H. is recipient of an ICREA Academia Award (Generalitat de Catalunya, Spain)
- dc.format.mimetype application/pdfca
- dc.identifier.citation Gaspa L, González-Medina A, Hidalgo E, Ayté J. A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave. Cell cycle. 2016; 15(5): 720-729. DOI 10.1080/15384101.2016.1148839ca
- dc.identifier.doi http://dx.doi.org/10.1080/15384101.2016.1148839
- dc.identifier.issn 1538-4101
- dc.identifier.uri http://hdl.handle.net/10230/26073
- dc.language.iso engca
- dc.publisher Taylor & Francis (Routledge)ca
- dc.relation.ispartof Cell cycle. 2016;15(5):720-9
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-31939
- dc.rights © Taylor & Francis. This is an electronic version of an article published in Gaspa L, González-Medina A, Hidalgo E, Ayté J. A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave. Cell cycle. 2016; 15(5): 720-729. DOI 10.1080/15384101.2016.1148839. Cell cycle is available online at: www.tandfonline.com http://www.tandfonline.com/doi/pdf/10.1080/15384101.2016.1148839.ca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.subject.keyword COP9
- dc.subject.keyword Cdc22-YFP
- dc.subject.keyword Elongator
- dc.subject.keyword G1/S transition
- dc.subject.keyword MBF complex
- dc.subject.keyword S. pombe
- dc.subject.other Schizosaccharomyces pombe -- Metabolismeca
- dc.subject.other ADNca
- dc.title A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional waveca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/acceptedVersionca