Secondary enhancers synergise with primary enhancers to guarantee fine-tuned muscle gene expression
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- dc.contributor.author Guerrero, Luciaca
- dc.contributor.author Marco Ferreres, Raquelca
- dc.contributor.author Serrano, Antonio L.ca
- dc.contributor.author Arredondo, Juan Joseca
- dc.contributor.author Cervera, Margaritaca
- dc.date.accessioned 2015-06-04T06:56:57Z
- dc.date.available 2015-06-04T06:56:57Z
- dc.date.issued 2010ca
- dc.description.abstract Although tight quantitative control of gene expression is required to ensure that organs and tissues function correctly, the transcriptional mechanisms underlying this process still remain poorly understood. Here, we describe novel and evolutionary conserved secondary enhancers that are needed for the regulation of the expression of Troponin I genes. Secondary enhancers are silent when tested individually in electroporated muscles but interact with the primary enhancers and are required to precisely control the appropriate timing, the tissue and fibre specificity, and the quantitative expression of these genes during muscle differentiation. Synergism is completely dependent of the fully conserved MEF2 site present on the primary enhancers core of skeletal muscle Troponin I genes. Thus, while each of these paired enhancers has a different function, the concerted action of both is crucial to recapitulate endogenous gene expression. Through comparative genomics, we predict that this mechanism has also arisen in other mammalian muscle genes. Our results reveal the existence of a novel mechanism, conserved from flies to mammals, to fine-tune gene expression in each muscle and probably other tissuesen
- dc.description.sponsorship This research was supported by grants BFU2004-05384, BFU2007-61711BMC from the DGICYT (Spanish Ministry of Education, Culture and Sports), FIS-PS09/01267, Marato-TV3 and by Fundación Mutua Madrileñaen
- dc.format.mimetype application/pdfca
- dc.identifier.citation Guerrero L, Marco-Ferreres R, Serrano AL, Arredondo JJ, Cervera M. Secondary enhancers synergise with primary enhancers to guarantee fine-tuned muscle gene expression. Developmental Biology. 2010;337(1):16-28. DOI: 10.1016/j.ydbio.2009.10.006ca
- dc.identifier.doi http://dx.doi.org/10.1016/j.ydbio.2009.10.006
- dc.identifier.issn 0012-1606ca
- dc.identifier.uri http://hdl.handle.net/10230/23728
- dc.language.iso engca
- dc.publisher Elsevierca
- dc.relation.ispartof Developmental Biology. 2010;337(1):16-28
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/BFU2004-05384
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/BFU2007-61711
- dc.rights © Elsevier This is the published version of an article http://dx.doi.org/10.1016/j.ydbio.2009.10.006 that appeared in the journal Developmental Biology. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-licenseen
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.subject.keyword Paired enhancersen
- dc.subject.keyword Secondary enhancersen
- dc.subject.keyword Fine-tuning gene-transcription controlen
- dc.subject.keyword Global transcription regulationen
- dc.subject.keyword Mouse fast, slow and cardiac Troponin I genesen
- dc.subject.other Ratolins (Animals de laboratori)ca
- dc.subject.other Genètica animalca
- dc.title Secondary enhancers synergise with primary enhancers to guarantee fine-tuned muscle gene expressionen
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca