Bacillus firmus strain I-1582, a nematode antagonist by itself and through the plant

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• dc.contributor.author Ghahremani, Zahra
• dc.contributor.author Escudero, Nuria
• dc.contributor.author Beltrán Anadón, Daniel
• dc.contributor.author Saus Martínez, Ester
• dc.contributor.author Cunquero, Marina
• dc.contributor.author Andilla, Jordi
• dc.contributor.author Loza-Alvarez, Pablo
• dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-
• dc.contributor.author Sorribas, F. Javier
• dc.date.accessioned 2020-10-21T06:39:43Z
• dc.date.available 2020-10-21T06:39:43Z
• dc.date.issued 2020
• dc.description.abstract Bacillus firmus I-1582 is approved in Europe for the management of Meloidogyne on vegetable crops. However, little information about its modes of action and temperature requirements is available, despite the effect of these parameters in its efficacy. The cardinal temperatures for bacterial growth and biofilm formation were determined. The bacteria was transformed with GFP to study its effect on nematode eggs and root colonization of tomato (Solanum lycopersicum) and cucumber (Cucumis sativus) by laser-scanning confocal microscopy. Induction of plant resistance was determined in split-root experiments and the dynamic regulation of genes related to jasmonic acid (JA) and salicylic acid (SA) by RT-qPCR at three different times after nematode inoculation. The bacteria was able to grow and form biofilms between 15 and 45°C; it degraded egg-shells and colonized eggs; it colonized tomato roots more extensively than cucumber roots; it induced systemic resistance in tomato, but not in cucumber; SA and JA related genes were primed at different times after nematode inoculation in tomato, but only the SA-related gene was up-regulated at 7 days after nematode inoculation in cucumber. In conclusion, B. firmus I-1582 is active at a wide range of temperatures; its optimal growth temperature is 35°C; it is able to degrade Meloidogyne eggs, and to colonize plant roots, inducing systemic resistance in a plant dependent species manner.
• dc.description.sponsorship Thanks are given to the Spanish Ministry of Economy and Competitiveness (MINECO) for funding the AGL2013-49040-C2-1-R project, to the Ministry of Science and Innovation from the Spanish Government for funding the AGL2017-89785-R, and to the European Regional Development Fund (FEDER). MC, JA, and PL-A acknowledges financial support from the Spanish Ministry of Economy and Competitiveness through the “Severo Ochoa” program for Centers of Excellence in R&D (SEV-2015-0522), from Fundació Privada Cellex, Fundación Mig-Puig and from Generalitat de Catalunya through the CERCA program and Laser lab Europe through the research and innovation programme under grant agreement No. 654148.
• dc.format.mimetype application/pdf
• dc.identifier.citation Ghahremani Z, Escudero N, Beltrán-Anadón D, Saus E, Cunquero M, Andilla J, Loza-Alvarez P, Gabaldón T, Sorribas FJ. Bacillus firmus strain I-1582, a nematode antagonist by itself and through the plant. Front Plant Sci. 2020; 11:796. DOI: 10.3389/fpls.2020.00796
• dc.identifier.doi http://dx.doi.org/10.3389/fpls.2020.00796
• dc.identifier.issn 1664-462X
• dc.identifier.uri http://hdl.handle.net/10230/45535
• dc.language.iso eng
• dc.publisher Frontiers
• dc.relation.ispartof Front Plant Sci. 2020; 11:796
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/654148
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/AGL2013-49040-C2-1-R
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/AGL2017-89785-R
• dc.rights © 2020 Ghahremani, Escudero, Beltrán-Anadón, Saus, Cunquero, Andilla, Loza-Alvarez, Gabaldón and Sorribas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Cucumis sativus
• dc.subject.keyword Meloidogyne incognita
• dc.subject.keyword Solanum lycopersicum
• dc.subject.keyword Induced resistance
• dc.subject.keyword Nematode antagonist
• dc.subject.keyword Root-knot nematodes
• dc.title Bacillus firmus strain I-1582, a nematode antagonist by itself and through the plant
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion