Human Oocyte-Derived Methylation Differences Persist in the Placenta Revealing Widespread Transient Imprinting

Sanchez-Delgado, Marta; Court, Franck; Vidal, Enrique; Medrano, Jose; Monteagudo-Sánchez, Ana; Martin-Trujillo, Alex; Tayama, Chiharu; Iglesias-Platas, Isabel; Kondova, Ivanela; Bontrop, Ronald E.; Poo-Llanillo, Maria Eugenia; Marquès i Bonet, Tomàs, 1975-; Nakabayashi, Kazuhiko; Simón, Carlos; Monk, David

Human Oocyte-Derived Methylation Differences Persist in the Placenta Revealing Widespread Transient Imprinting

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dc.contributor.author Sanchez-Delgado, Martaca
dc.contributor.author Court, Franckca
dc.contributor.author Vidal, Enriqueca
dc.contributor.author Medrano, Joseca
dc.contributor.author Monteagudo-Sánchez, Anaca
dc.contributor.author Martin-Trujillo, Alexca
dc.contributor.author Tayama, Chiharuca
dc.contributor.author Iglesias-Platas, Isabelca
dc.contributor.author Kondova, Ivanelaca
dc.contributor.author Bontrop, Ronald E.ca
dc.contributor.author Poo-Llanillo, Maria Eugeniaca
dc.contributor.author Marquès i Bonet, Tomàs, 1975-ca
dc.contributor.author Nakabayashi, Kazuhikoca
dc.contributor.author Simón, Carlosca
dc.contributor.author Monk, Davidca
dc.date.accessioned 2017-03-06T13:52:04Z
dc.date.available 2017-03-06T13:52:04Z
dc.date.issued 2016
dc.description.abstract Thousands of regions in gametes have opposing methylation profiles that are largely resolved during the post-fertilization epigenetic reprogramming. However some specific sequences associated with imprinted loci survive this demethylation process. Here we present the data describing the fate of germline-derived methylation in humans. With the exception of a few known paternally methylated germline differentially methylated regions (DMRs) associated with known imprinted domains, we demonstrate that sperm-derived methylation is reprogrammed by the blastocyst stage of development. In contrast a large number of oocyte-derived methylation differences survive to the blastocyst stage and uniquely persist as transiently methylated DMRs only in the placenta. Furthermore, we demonstrate that this phenomenon is exclusive to primates, since no placenta-specific maternal methylation was observed in mouse. Utilizing single cell RNA-seq datasets from human preimplantation embryos we show that following embryonic genome activation the maternally methylated transient DMRs can orchestrate imprinted expression. However despite showing widespread imprinted expression of genes in placenta, allele-specific transcriptional profiling revealed that not all placenta-specific DMRs coordinate imprinted expression and that this maternal methylation may be absent in a minority of samples, suggestive of polymorphic imprinted methylation.
dc.description.sponsorship This work was supported by Spanish Ministerio de Educación y Competitividad (MINECO) (BFU2014-53093 to DM) co-funded with the European Union Regional Development Fund (FEDER). AMS is a recipient of a FPI PhD studentship from MINECO. TMB is supported by ICREA, EMBO YIP 2013, MINECO BFU2014-55090-P (FEDER), BFU2015-7116-ERC and BFU2015-6215-ERCU01 MH106874 grant, Fundacio Zoo Barcelona and Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya.
dc.format.mimetype application/pdfca
dc.identifier.citation Sanchez-Delgado M, Court F, Vidal E, Medrano J, Monteagudo-Sánchez A, Martin-Trujillo A, Tayama C, Iglesias-Platas I, Kondova I, Bontrop R, Poo-Llanillo ME, Marques i Bonet T, Nakabayashi K, Simón C, Monk D. Human Oocyte-Derived Methylation Differences Persist in the Placenta Revealing Widespread Transient Imprinting. PLoS Genetics. 2016; 12(11): e1006427. DOI: 10.1371/journal.pgen.1006427
dc.identifier.doi http://dx.doi.org/10.1371/journal.pgen.1006427
dc.identifier.issn 1553-7390
dc.identifier.uri http://hdl.handle.net/10230/28175
dc.language.iso eng
dc.publisher Public Library of Science (PLoS)ca
dc.relation.ispartof PLoS Genetics. 2016; 12(11): e1006427
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-55090-P
dc.rights © 2016 Sanchez-Delgado et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject.keyword DNA methylation
dc.subject.keyword Placenta
dc.subject.keyword Genomic imprinting
dc.subject.keyword Blastocysts
dc.subject.keyword Polymerase chain reaction
dc.subject.keyword Embryos
dc.subject.keyword Oocytes
dc.subject.keyword Sperm
dc.title Human Oocyte-Derived Methylation Differences Persist in the Placenta Revealing Widespread Transient Imprintingca
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

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