Binding of the transcription factor Atf1 to promoters serves as a barrier to phase nucleosome arrays and avoid cryptic transcription

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• dc.contributor.author García, Patríciaca
• dc.contributor.author Paulo Mirasol, Esther, 1984-ca
• dc.contributor.author Gao, Junca
• dc.contributor.author Wahls, Wayne Pca
• dc.contributor.author Ayté del Olmo, Joséca
• dc.contributor.author Lowy Gallego, Ernestoca
• dc.contributor.author Hidalgo Hernando, Elenaca
• dc.date.accessioned 2015-04-08T10:14:39Z
• dc.date.available 2015-04-08T10:14:39Z
• dc.date.issued 2014ca
• dc.description.abstract Schizosaccharomyces pombe displays a large transcriptional response common to several stress conditions, regulated primarily by the transcription factor Atf1. Atf1-dependent promoters contain especially broad nucleosome depleted regions (NDRs) prior to stress imposition. We show here that basal binding of Atf1 to these promoters competes with histones to create wider NDRs at stress genes. Moreover, deletion of atf1 results in nucleosome disorganization specifically at stress coding regions and derepresses antisense transcription. Our data indicate that the transcription factor binding to promoters acts as an effective barrier to fix the +1 nucleosome and phase downstream nucleosome arrays to prevent cryptic transcription.en
• dc.description.sponsorship Spanish Ministry of Science and Innovation [BFU2012-32045]; PLAN E and FEDER; Generalitat de Catalunya (Spain) [SGR2009-195 to E.H.]; ICREA Academia Awards (Generalitat de Catalunya) [to J.A. and E.H.]; Juan de la Cierva from the Spanish Ministry of Science and Innovation[toP.G.]. Funding for open access charge: Spanish Ministry of Science and Innovation [BFU2012-32045]; PLAN E and FEDER; Generalitat de Catalunya (Spain) [SGR2009-195 to E.H.]en
• dc.format.mimetype application/pdfca
• dc.identifier.citation Garcia P, Paulo E, Gao J, Wahls WP, Ayté J, Lowy E, Hidalgo E. Binding of the transcription factor Atf1 to promoters serves as a barrier to phase nucleosome arrays and avoid cryptic transcription. Nucleic Acids Research. 2014; 42(16): 10351-10359. DOI 10.1093/nar/gku704ca
• dc.identifier.doi http://dx.doi.org/10.1093/nar/gku704
• dc.identifier.issn 0305-1048ca
• dc.identifier.uri http://hdl.handle.net/10230/23351
• dc.language.iso engca
• dc.publisher Oxford University Pressca
• dc.relation.ispartof Nucleic Acids Research. 2014;42(16):10351-9
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-32045
• dc.rights © Garcia P, Paulo E, Gao J, Wahls WP, Ayté J, Lowy E, Hidalgo E [2014]. Published by Oxford University Press. This is an Open Access article distributed under the terms of a Creative Commons Attribution Licenseca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.other Estrès oxidatiuca
• dc.subject.other Schizosaccharomyces pombe -- Metabolismeca
• dc.title Binding of the transcription factor Atf1 to promoters serves as a barrier to phase nucleosome arrays and avoid cryptic transcriptionen
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca