RNA polymerase errors cause splicing defects and can be regulated by differential expression of RNA polymerase subunits
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- dc.contributor.author Carey, Lucas, 1980-ca
- dc.date.accessioned 2016-02-10T13:11:52Z
- dc.date.available 2016-02-10T13:11:52Z
- dc.date.issued 2015
- dc.description.abstract Errors during transcription may play an important role in determining cellular phenotypes: the RNA polymerase error rate is >4 orders of magnitude higher than that of DNA polymerase and errors are amplified >1000-fold due to translation. However, current methods to measure RNA polymerase fidelity are low-throughout, technically challenging, and organism specific. Here I show that changes in RNA polymerase fidelity can be measured using standard RNA sequencing protocols. I find that RNA polymerase is error-prone, and these errors can result in splicing defects. Furthermore, I find that differential expression of RNA polymerase subunits causes changes in RNA polymerase fidelity, and that coding sequences may have evolved to minimize the effect of these errors. These results suggest that errors caused by RNA polymerase may be a major source of stochastic variability at the level of single cells.ca
- dc.description.sponsorship Agència de Gestió d’Ajuts Universitaris i de Recerca 2014 SGR 0974.
- dc.format.mimetype application/pdfca
- dc.identifier.citation Carey LB. RNA polymerase errors cause splicing defects and can be regulated by differential expression of RNA polymerase subunits. eLife. 2015;4:e09945. DOI: 10.7554/eLife.09945ca
- dc.identifier.doi http://dx.doi.org/10.7554/eLife.09945
- dc.identifier.issn 2050-084X
- dc.identifier.uri http://hdl.handle.net/10230/25767
- dc.language.iso engca
- dc.publisher eLifeca
- dc.relation.ispartof eLife. 2015;4:e09945
- dc.rights © 2015 Carey. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.ca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/ca
- dc.subject.keyword RNA polymerase
- dc.subject.keyword RNA-seq
- dc.subject.keyword S. cerevisiae
- dc.subject.keyword Biological errors
- dc.subject.keyword Computational biology
- dc.subject.keyword Evolutionary biology
- dc.subject.keyword Genomics
- dc.subject.keyword Human
- dc.subject.keyword Splicing
- dc.subject.keyword Systems biology
- dc.subject.other RNA-polimerasesca
- dc.title RNA polymerase errors cause splicing defects and can be regulated by differential expression of RNA polymerase subunitsca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca