Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion
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- dc.contributor.author Faria, Melissa
- dc.contributor.author Prats, Eva
- dc.contributor.author Rosas Ramírez, Jonathan Ricardo
- dc.contributor.author Bellot, Marina
- dc.contributor.author Bedrossiantz, Juliette
- dc.contributor.author Pagano, Maria
- dc.contributor.author Valls, Arnau
- dc.contributor.author Gómez Canela, Cristian
- dc.contributor.author Porta, Josep M.
- dc.contributor.author Mestres i López, Jordi
- dc.contributor.author Garcia Reyero, Natalia
- dc.contributor.author Faggio, Caterina
- dc.contributor.author Gómez Oliván, Leobardo Manuel
- dc.contributor.author Raldua, Demetrio
- dc.date.accessioned 2021-07-12T06:09:06Z
- dc.date.available 2021-07-12T06:09:06Z
- dc.date.issued 2021
- dc.description.abstract Fenitrothion is an organophosphorus insecticide usually found in aquatic ecosystems at concentrations in the range of low ng/L. In this manuscript we show that 24 h exposure to environmental concentrations of fenitrothion, from ng/L to low μg/L, altered basal locomotor activity, visual-motor response and acoustic/vibrational escape response of zebrafish larvae. Furthermore, fenitrothion and expression of gap43a, gfap, atp2b1a, and mbp exhibited a significant non-monotonic concentration-response relationship. Once determined that environmental concentrations of fenitrothion were neurotoxic for zebrafish larvae, a computational analysis identified potential protein targets of this compound. Some of the predictions, including interactions with acetylcholinesterase, monoamine-oxidases and androgen receptor (AR), were experimentally validated. Binding to AR was the most suitable candidate for molecular initiating event, as indicated by both the up-regulation of cyp19a1b and sult2st3 and the non-monotonic relationship found between fenitrothion and the observed responses. Finally, when the integrity of the monoaminergic system was evaluated, altered levels of L-DOPA, DOPAC, HVA and 5-HIAA were found, as well as a significant up-regulation of slc18a2 expression at the lowest concentrations of fenitrothion. These data strongly suggest that concentrations of fenitrothion commonly found in aquatic ecosystems present a significant environmental risk for fish communities.
- dc.format.mimetype application/pdf
- dc.identifier.citation Faria M, Prats E, Rosas Ramírez JR, Bellot M, Bedrossiantz J, Pagano M, Valls A, Gomez-Canela C, Porta JM, Mestres J, Garcia-Reyero N, Faggio C, Gómez Oliván LM, Raldua D. Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion. Sci Total Environ. 2021;775:145671. DOI: 10.1016/j.scitotenv.2021.145671
- dc.identifier.doi http://dx.doi.org/10.1016/j.scitotenv.2021.145671
- dc.identifier.issn 0048-9697
- dc.identifier.uri http://hdl.handle.net/10230/48152
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof Sci Total Environ. 2021;775:145671
- dc.rights © 2021 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Acetylcholinesterase inhibitor
- dc.subject.keyword Androgenic effects
- dc.subject.keyword Endocrine disruptors
- dc.subject.keyword Neurotoxicity
- dc.subject.keyword Predicted target profile
- dc.subject.keyword Risk assessment
- dc.title Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion