Spatial control of lipid droplet proteins by the ERAD ubiquitin ligase Doa10

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• dc.contributor.author Ruggiano, Annamaria, 1985-ca
• dc.contributor.author Mora, Gabrielca
• dc.contributor.author Buxó, Lauraca
• dc.contributor.author Carvalho, Pedro C.ca
• dc.date.accessioned 2017-04-20T12:09:17Z
• dc.date.available 2017-04-20T12:09:17Z
• dc.date.issued 2016
• dc.description.abstract The endoplasmic reticulum (ER) plays a central role in the biogenesis of most membrane proteins. Among these are proteins localized to the surface of lipid droplets (LDs), fat storage organelles delimited by a phospholipid monolayer. The LD monolayer is often continuous with the membrane of the ER allowing certain membrane proteins to diffuse between the two organelles. In these connected organelles, how some proteins concentrate specifically at the surface of LDs is not known. Here, we show that the ERAD ubiquitin ligase Doa10 controls the levels of some LD proteins. Their degradation is dependent on the localization to the ER and appears independent of the folding state. Moreover, we show that by degrading the ER pool of these LD proteins, ERAD contributes to restrict their localization to LDs. The signals for LD targeting and Doa10‐mediated degradation overlap, indicating that these are competing events. This spatial control of protein localization is a novel function of ERAD that might contribute to generate functional diversity in a continuous membrane system.
• dc.description.sponsorship A. Ruggiano was supported by a “La Caixa” graduate fellowship; P. Carvalho is supported by CRG, an International Early Career Award from the HHMI, the EMBO Young Investigator Program, and grants from the Spanish MCCIN and ERC (FP7/2007‐2013 ERC grant agreement no. 309477 DROPFAT). We acknowledge support of the Spanish Ministry of Economy and Competitiveness, “Centro de Excelencia Severo Ochoa 2013–2017”, SEV‐2012‐0208.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Ruggiano A, Mora G, Buxó L, Carvalho P. Spatial control of lipid droplet proteins by the ERAD ubiquitin ligase Doa10. EMBO Journal. 2016;35(15):1597-719. DOI: 10.15252/embj.201593106
• dc.identifier.doi http://dx.doi.org/10.15252/embj.201593106
• dc.identifier.issn 0261-4189
• dc.identifier.uri http://hdl.handle.net/10230/30860
• dc.language.iso eng
• dc.publisher EMBO Pressca
• dc.relation.ispartof EMBO Journal. 2016;35(15):1597-719
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/309477
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SEV2012-0208
• dc.rights This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Doa10
• dc.subject.keyword Endoplasmic reticulum
• dc.subject.keyword ERAD
• dc.subject.keyword Lipid droplets
• dc.subject.keyword Protein degradation
• dc.subject.keyword
• dc.title Spatial control of lipid droplet proteins by the ERAD ubiquitin ligase Doa10ca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion