Genome-wide screening of regulators of catalase expression: role of a transcription complex and histone and tRNA modification complexes on adaptation to stress
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- dc.contributor.author García Rodríguez, Patriciaca
- dc.contributor.author Encinar del Dedo, Javierca
- dc.contributor.author Ayté del Olmo, Joséca
- dc.contributor.author Hidalgo Hernando, Elenaca
- dc.date.accessioned 2016-01-13T16:09:01Z
- dc.date.available 2017-01-08T03:00:03Z
- dc.date.issued 2016
- dc.description.abstract In response to environmental cues, the mitogen-activated protein kinase Sty1-driven signaling cascade activates hundreds of genes to induce a robust anti-stress cellular response in fission yeast. Thus, upon stress imposition Sty1 transiently accumulates in the nucleus where it up-regulates transcription through the Atf1 transcription factor. Several regulators of transcription and translation have been identified as important to mount an integral response to oxidative stress, such as the Spt-Ada-Gcn5-acetyl transferase or Elongator complexes, respectively. With the aim of identifying new regulators of this massive gene expression program, we have used a GFP-based protein reporter and screened a fission yeast deletion collection using flow cytometry. We find that the levels of catalase fused to GFP, both before and after a threat of peroxides, are altered in hundreds of strains lacking components of chromatin modifiers, transcription complexes, and modulators of translation. Thus, the transcription elongation complex Paf1, the histone methylase Set1-COMPASS, and the translation-related Trm112 dimers are all involved in full expression of Ctt1-GFP and in wild-type tolerance to peroxidesca
- dc.description.sponsorship This work was supported by the Spanish Ministry of Science and Innovation (BFU2012-32045 (to E.H.) and BFU2012-31939 (to J.A.), PLAN E and FEDER, and by 2014-SGR-154 from Generalitat de Catalunya (Spain) (to E.H. and J.A.).
- dc.format.mimetype application/pdfca
- dc.identifier.citation García P, Encinar Del Dedo J, Ayté J, Hidalgo E. Genome-wide screening of regulators of catalase expression: role of a transcription complex and histone and tRNA modification complexes on adaptation to stress. J Biol Chem. 2016;291(2):790-9. DOI: 10.1074/jbc.M115.696658ca
- dc.identifier.doi http://dx.doi.org/10.1074/jbc.M115.696658
- dc.identifier.issn 0021-9258
- dc.identifier.uri http://hdl.handle.net/10230/25571
- dc.language.iso engca
- dc.publisher American Society for Biochemistry and Molecular Biology (ASBMB)ca
- dc.relation.ispartof Journal of biological chemistry. 2016;291(2):790-9
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-32045
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-31939
- dc.rights © American Society for Biochemistry and Molecular Biology (ASBMB) /nThis research was originally published in Journal of biological chemistry. García P, Encinar Del Dedo J, Ayté J, Hidalgo E. Genome-wide screening of regulators of catalase expression: role of a transcription complex and histone and tRNA modification complexes on adaptation to stress. Journal of biological chemistry. 2016; 291(2): 790-799ca
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword Stress response
- dc.subject.keyword Gene expression
- dc.subject.keyword Catalase
- dc.subject.keyword tRNA
- dc.subject.keyword Schizosaccharomyces pombe
- dc.subject.other Histonesca
- dc.title Genome-wide screening of regulators of catalase expression: role of a transcription complex and histone and tRNA modification complexes on adaptation to stressca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/acceptedVersionca