Analysis of Vibrio harveyi adaptation in sea water microcosms at elevated temperature provides insights into the putative mechanisms of its persistence and spread in the time of global warming

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  • dc.contributor.author Montánchez, Itxaso
  • dc.contributor.author Ogayar, Elixabet
  • dc.contributor.author Hernández Plágaro, Ander
  • dc.contributor.author Esteve-Codina, Anna
  • dc.contributor.author Gómez-Garrido, Jèssica
  • dc.contributor.author Orruño, Maite
  • dc.contributor.author Arana, Inés
  • dc.contributor.author Kaberdin, Vladimir R.
  • dc.date.accessioned 2019-03-18T08:38:07Z
  • dc.date.available 2019-03-18T08:38:07Z
  • dc.date.issued 2019
  • dc.description.abstract Discovering the means to control the increasing dissemination of pathogenic vibrios driven by recent climate change is challenged by the limited knowledge of the mechanisms in charge of Vibrio spp. persistence and spread in the time of global warming. To learn about physiological and gene expression patterns associated with the long-term persistence of V. harveyi at elevated temperatures, we studied adaptation of this marine bacterium in seawater microcosms at 30 °C which closely mimicked the upper limit of sea surface temperatures around the globe. We found that nearly 90% of cells lost their culturability and became partly damaged after two weeks, thus suggesting a negative impact of the combined action of elevated temperature and shortage of carbon on V. harveyi survival. Moreover, further gene expression analysis revealed that major adaptive mechanisms were poorly coordinated and apparently could not sustain cell fitness. On the other hand, elevated temperature and starvation promoted expression of many virulence genes, thus potentially reinforcing the pathogenicity of this organism. These findings suggest that the increase in disease outbreaks caused by V. harveyi under rising sea surface temperatures may not reflect higher cell fitness, but rather an increase in virulence enabling V. harveyi to escape from adverse environments to nutrient rich, host-pathogen associations.
  • dc.description.sponsorship This work was supported by the Spanish Ministry of Economy and Competitiveness grant CGL2015-70929-R, Ikerbasque (Basque Foundation for Science) and pre-doctoral grants PRE-2013-1-901 (I.M.) from the Basque Government and PIF15/101 (E.O.) from the University of the Basque Country. A.E.-C. is funded by ISCIII of the MINECO (Ref: PT17/0009/0019) and cofinanced by FEDER.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Montánchez I, Ogayar E, Plágaro AH, Esteve-Codina A, Gómez-Garrido J, Orruño M, Arana I, Kaberdin VR. Analysis of Vibrio harveyi adaptation in sea water microcosms at elevated temperature provides insights into the putative mechanisms of its persistence and spread in the time of global warming. Sci Rep. 2019;9(1):289. DOI: 10.1038/s41598-018-36483-0
  • dc.identifier.doi http://dx.doi.org/10.1038/s41598-018-36483-0
  • dc.identifier.issn 2045-2322
  • dc.identifier.uri http://hdl.handle.net/10230/36850
  • dc.language.iso eng
  • dc.publisher Nature Research
  • dc.relation.ispartof Scientific Reports. 2019; 9(1):289
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/CGL2015-70929-R
  • dc.rights © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Microbial ecology
  • dc.subject.keyword Water microbiology
  • dc.title Analysis of Vibrio harveyi adaptation in sea water microcosms at elevated temperature provides insights into the putative mechanisms of its persistence and spread in the time of global warming
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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