Mitotic cell rounding and epithelial thinning regulate lumen growth and shape

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• dc.contributor.author Hoijman, Estebanca
• dc.contributor.author Rubbini, Davide, 1985-ca
• dc.contributor.author Colombelli, Julienca
• dc.contributor.author Alsina i Español, Bertaca
• dc.date.accessioned 2016-02-24T14:27:49Z
• dc.date.available 2016-02-24T14:27:49Z
• dc.date.issued 2015
• dc.description.abstract Many organ functions rely on epithelial cavities with particular shapes. Morphogenetic anomalies in these cavities lead to kidney, brain or inner ear diseases. Despite their relevance, the mechanisms regulating lumen dimensions are poorly understood. Here, we perform live imaging of zebrafish inner ear development and quantitatively analyse the dynamics of lumen growth in 3D. Using genetic, chemical and mechanical interferences, we identify two new morphogenetic mechanisms underlying anisotropic lumen growth. The first mechanism involves thinning of the epithelium as the cells change their shape and lose fluids in concert with expansion of the cavity, suggesting an intra-organ fluid redistribution process. In the second mechanism, revealed by laser microsurgery experiments, mitotic rounding cells apicobasally contract the epithelium and mechanically contribute to expansion of the lumen. Since these mechanisms are axis specific, they not only regulate lumen growth but also the shape of the cavity.ca
• dc.description.sponsorship This work has been supported by grants BFU2011-27006 from MICINN and XVICN from Fundación Areces to B.A.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Hoijman E, Rubbini D, Colombelli J, Alsina B. Mitotic cell rounding and epithelial thinning regulate lumen growth and shape. Nature communications. 2015;6:7355. DOI 10.1038/ncomms8355ca
• dc.identifier.doi http://dx.doi.org/10.1038/ncomms8355
• dc.identifier.issn 2041-1723
• dc.identifier.uri http://hdl.handle.net/10230/25942
• dc.language.iso engca
• dc.publisher Nature Publishing Groupca
• dc.relation.ispartof Nature communications. 2015;6:7355
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2011-27006
• dc.rights © Nature Publishing Group. http://www.nature.com/ncomms/2015/150616/ncomms8355/pdf/ncomms8355.pdf/nCreative Commons Attribution Non-Commercial No Derivatives License and Creative Commons Attribution Non-Commercial Share Alike Licenseca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.subject.other Cèl·lules epitelialsca
• dc.subject.other Peixos zebra (Animals de laboratori)ca
• dc.title Mitotic cell rounding and epithelial thinning regulate lumen growth and shapeca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca