A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress

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• dc.contributor.author Viéitez Manrique, Cristina, 1984-
• dc.contributor.author Martínez Cebrián, Gerard, 1992-
• dc.contributor.author Solé, Carme
• dc.contributor.author Böttcher, René
• dc.contributor.author Potel, Clement M.
• dc.contributor.author Savitski, Mikhail M.
• dc.contributor.author Onnebo, Sara
• dc.contributor.author Fabregat, Marc
• dc.contributor.author Shilatifard, Ali
• dc.contributor.author Posas Garriga, Francesc
• dc.contributor.author Nadal Clanchet, Eulàlia de
• dc.date.accessioned 2020-05-06T07:10:36Z
• dc.date.available 2020-05-06T07:10:36Z
• dc.date.issued 2020
• dc.description.abstract Cells have the ability to sense, respond and adapt to environmental fluctuations. Stress causes a massive reorganization of the transcriptional program. Many examples of histone post-translational modifications (PTMs) have been associated with transcriptional activation or repression under steady-state growth conditions. Comparatively less is known about the role of histone PTMs in the cellular adaptive response to stress. Here, we performed high-throughput genetic screenings that provide a novel global map of the histone residues required for transcriptional reprogramming in response to heat and osmotic stress. Of note, we observed that the histone residues needed depend on the type of gene and/or stress, thereby suggesting a 'personalized', rather than general, subset of histone requirements for each chromatin context. In addition, we identified a number of new residues that unexpectedly serve to regulate transcription. As a proof of concept, we characterized the function of the histone residues H4-S47 and H4-T30 in response to osmotic and heat stress, respectively. Our results uncover novel roles for the kinases Cla4 and Ste20, yeast homologs of the mammalian PAK2 family, and the Ste11 MAPK as regulators of H4-S47 and H4-T30, respectively. This study provides new insights into the role of histone residues in transcriptional regulation under stress conditions.
• dc.description.sponsorship FPI predoctoral fellowships (to G.M.-C., C.V.); Juan de la Cierva post-doctoral fellowship (to R.B.); Spanish Ministry of Economy and Competitiveness [BFU2017-85152-P, FEDER to E.N., PGC2018-094136-B-I00 and FEDER to F.P.); Catalan Government [2017 SGR 799 to E.N. and F.P.]; Unidad de Excelencia Maria de Maeztu [MDM-2014-0370 to the UPF]; F.P. is a recipient of an ICREA Acadèmia (Catalan Government); Spanish Ministry of Economy, Industry and Competitiveness (MINECO) through the Centres of Excellence Severo Ochoa award; CERCA Programme of the Catalan Government; M.M.S. was supported by the European Molecular Biology Laboratory (EMBL); C.M.P. was supported by a fellowship from the EMBL Interdisciplinary Postdoc (EI3POD) Programme under Marie Skłodowska‐Curie Actions COFUND. Funding for open access charge: Spanish Ministry of Economy and Competitiveness [BFU2017-85152-P, FEDER to E.N.].
• dc.format.mimetype application/pdf
• dc.identifier.citation Viéitez C, Martínez-Cebrián G, Solé C, Böttcher R, Potel CM, Savitski MM, Onnebo S, Fabregat M, Shilatifard A, Posas F, de Nadal E. A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress. Nucleic Acids Res. 2020; 48(7):3455-75. DOI: 10.1093/nar/gkaa081
• dc.identifier.doi http://dx.doi.org/10.1093/nar/gkaa081
• dc.identifier.issn 0305-1048
• dc.identifier.uri http://hdl.handle.net/10230/44422
• dc.language.iso eng
• dc.publisher Oxford University Press
• dc.relation.ispartof Nucleic Acids Res. 2020; 48(7):3455-75
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/BFU2017-85152-P
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-094136-B-I00
• dc.rights © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.title A genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion