DIA+: a data-independent acquisition method combining multiple precursor charges to improve peptide signal
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Borràs, Eva
- dc.contributor.author Sabidó Aguadé, Eduard, 1981-
- dc.date.accessioned 2018-11-16T10:38:40Z
- dc.date.available 2018-11-16T10:38:40Z
- dc.date.issued 2018
- dc.description.abstract Data-independent acquisition methods that acquire fragment ions from virtually any peptide in a sample have expanded the benefits of low-throughput targeted proteomics to proteome-wide analyses. While these methods have increased the reproducibility of peptide quantification across multiple samples, their sensitivity is still limited, and the quantification of complete proteomes remains a challenge. Here we present DIA+, a DIA method that combines signals from identical peptides with different charge states, resulting in improved signal-to-noise, additional number of fragments, and therefore in a higher number of identified and quantified peptides in complex samples.
- dc.description.sponsorship The CRG/UPF Proteomics Unit is a member of the ProteoRed PRB3 Consortium which is supported by Grant PT17/0019 of the PE I+D+i 2013-2016 from the Instituto de Salud Carlos III (ISCIII) and ERDF. We acknowledge support from the Spanish Ministry of Economy and Competitiveness, “Centro de Excelencia Severo Ochoa 2013-2017”, Grant SEV-2012-0208, and “Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya” (Grant 2017SGR595)
- dc.format.mimetype application/pdf
- dc.identifier.citation Borràs E, Sabidó E. DIA+: a data-independent acquisition method combining multiple precursor charges to improve peptide signal. Anal Chem. 2018 Nov 6; 90(21): 12339-12341. DOI: 10.1021/acs.analchem.8b03418
- dc.identifier.doi http://dx.doi.org/10.1021/acs.analchem.8b03418
- dc.identifier.issn 0003-2700
- dc.identifier.uri http://hdl.handle.net/10230/35779
- dc.language.iso eng
- dc.publisher American Chemical Society (ACS)
- dc.rights This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.analchem.8b03418
- dc.rights.accessRights info:eu-repo/semantics/embargoedAccess
- dc.subject.other Proteòmica
- dc.subject.other Proteïnes
- dc.subject.other Pèptids
- dc.title DIA+: a data-independent acquisition method combining multiple precursor charges to improve peptide signal
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/acceptedVersion