Exploring the epitranscriptome by native RNA sequencing
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- dc.contributor.author Begik, Oguzhan
- dc.contributor.author Mattick, John S.
- dc.contributor.author Novoa, Eva Maria
- dc.date.accessioned 2023-01-24T07:20:32Z
- dc.date.available 2023-01-24T07:20:32Z
- dc.date.issued 2022
- dc.description.abstract Chemical RNA modifications, collectively referred to as the "epitranscriptome," are essential players in fine-tuning gene expression. Our ability to analyze RNA modifications has improved rapidly in recent years, largely due to the advent of high-throughput sequencing methodologies, which typically consist of coupling modification-specific reagents, such as antibodies or enzymes, to next-generation sequencing. Recently, it also became possible to map RNA modifications directly by sequencing native RNAs using nanopore technologies, which has been applied for the detection of a number of RNA modifications, such as N6-methyladenosine (m6A), pseudouridine (Ψ), and inosine (I). However, the signal modulations caused by most RNA modifications are yet to be determined. A global effort is needed to determine the signatures of the full range of RNA modifications to avoid the technical biases that have so far limited our understanding of the epitranscriptome.
- dc.description.sponsorship This work was supported by the Spanish Ministry of Economy, Industry and Competitiveness (MEIC) (PID2021-128193NB-100 to E.M.N.), the European Research Council (ERC-2021-STG no. 101042103 to E.M.N.), the AECC Scientific Foundation (LABAE211658NOVO to E.M.N.), and UNSW Sydney (SHARP Professorial Fellowship to J.S.M.). We acknowledge the support of the MEIC to the EMBL partnership, Centro de Excelencia Severo Ochoa and CERCA Programme/Generalitat de Catalunya.
- dc.format.mimetype application/pdf
- dc.identifier.citation Begik O, Mattick JS, Novoa EM. Exploring the epitranscriptome by native RNA sequencing. RNA. 2022 Nov;28(11):1430-9. DOI: 10.1261/rna.079404.122
- dc.identifier.doi http://dx.doi.org/10.1261/rna.079404.122
- dc.identifier.issn 1355-8382
- dc.identifier.uri http://hdl.handle.net/10230/55416
- dc.language.iso eng
- dc.publisher Cold Spring Harbor Laboratory Press (CSHL Press)
- dc.relation.ispartof RNA. 2022 Nov;28(11):1430-9
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2021-128193NB-100
- dc.rights © 2022 Begik et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society. This article, published in RNA, is available undera Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
- dc.subject.keyword RNA modifications
- dc.subject.keyword Epitranscriptomics
- dc.subject.keyword Nanopore sequencing
- dc.subject.keyword Native RNA sequencing
- dc.title Exploring the epitranscriptome by native RNA sequencing
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion