The Ol1mpiad: concordance of behavioural faculties of stage 1 and stage 3 Drosophila larvae

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  • dc.contributor.author Almeida Carvalho, Maria J.ca
  • dc.contributor.author Braun, Andreas, 1983-ca
  • dc.contributor.author Louis, Matthieuca
  • dc.contributor.author Zlatic, Martaca
  • dc.date.accessioned 2018-06-15T07:49:59Z
  • dc.date.issued 2017
  • dc.description.abstract Mapping brain function to brain structure is a fundamental task for neuroscience. For such an endeavour, the Drosophila larva is simple enough to be tractable, yet complex enough to be interesting. It features about 10,000 neurons and is capable of various taxes, kineses and Pavlovian conditioning. All its neurons are currently being mapped into a light-microscopical atlas, and Gal4 strains are being generated to experimentally access neurons one at a time. In addition, an electron microscopic reconstruction of its nervous system seems within reach. Notably, this electron microscope-based connectome is being drafted for a stage 1 larva - because stage 1 larvae are much smaller than stage 3 larvae. However, most behaviour analyses have been performed for stage 3 larvae because their larger size makes them easier to handle and observe. It is therefore warranted to either redo the electron microscopic reconstruction for a stage 3 larva or to survey the behavioural faculties of stage 1 larvae. We provide the latter. In a community-based approach we called the Ol1mpiad, we probed stage 1 Drosophila larvae for free locomotion, feeding, responsiveness to substrate vibration, gentle and nociceptive touch, burrowing, olfactory preference and thermotaxis, light avoidance, gustatory choice of various tastants plus odour-taste associative learning, as well as light/dark-electric shock associative learning. Quantitatively, stage 1 larvae show lower scores in most tasks, arguably because of their smaller size and lower speed. Qualitatively, however, stage 1 larvae perform strikingly similar to stage 3 larvae in almost all cases. These results bolster confidence in mapping brain structure and behaviour across developmental stages.
  • dc.description.sponsorship The Gulbenkian/Melbourne group was supported by the Fundação para a Ciência e a Tecnologia (FCT; post-doctoral fellowship to M.J.A.-C.: SFRH/BPD/75993/2011; exploratory grant to M.J.A.-C. and C.K.M.: EXPL/BEX-BID/0497/2013). The Münster group was supported by the Cluster of Excellence Cells in Motion (CiM) and the CiM International Max Planck research school (CiM-IMPRS). The Barcelona group was supported by the Spanish Ministry of Economy and Competitiveness, 'Centro de Excelencia Severo Ochoa 2013-2017' (SEV-2012-0208), the CERCA Programme/Generalitat de Catalunya, the 'la Caixa' International PhD Programme, and the Spanish Ministry of Science and Innovation (BFU2011-26208). The LIN-GoLM group received support from the Wissenschaftsgemeinschaft Gottfried Wilhelm Leibniz, the State of Sachsen-Anhalt, the Center for Behavioral Brain Sciences Magdeburg and the Otto von Guericke Universität Magdeburg, as well as from the Deutsche Forschungsgemeinschaft (CRC 779 Motivated behaviour: B11; GE1091/4-1) and the European Commission (FP7-ICT project Miniature Insect Model for Active Learning MINIMAL). The Janelia group received support from the Howard Hughes Medical Institute. The Fribourg group was supported by a starter grant from the European Research Council (ERC-2012-StG 309832-PhotoNaviNet) and the Swiss National Science Foundation (31003A_169993). The Hamburg group was supported by the Deutsche Forschungsgemeinschaft (SPP 1926, Next generation optogenetics, SO1337/2-1), and the Landesforschungsförderung Hamburg (LFF-FV27). The LIN-MolSysBiol group received support from the Wissenschaftsgemeinschaft Gottfried Wilhelm Leibniz, the State of Sachsen-Anhalt, the Center for Behavioral Brain Sciences Magdeburg and the Deutsche Forschungsgemeinschaft (CRC 779 Motivated behaviour: B15; YA272/2-1). The Bonn group was supported by the Deutsche Forschungsgemeinschaft (PA 787/7-1) and Cluster of Excellence ImmunoSensation. The Konstanz group was supported by Deutsche Forschungsgemeinschaft (TH1584/1-1, TH1584/3-1), the Baden-Württemberg Stiftung and the Zukunftskolleg of the University of Konstanz.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Almeida-Carvalho MJ, Berh D, Braun A, Chen YC, Eichler K, Eschbach C et al. The Ol1mpiad: concordance of behavioural faculties of stage 1 and stage 3 Drosophila larvae. J Exp Biol. 2017 Jul 1;220(Pt 13):2452-2475. DOI: 10.1242/jeb.156646
  • dc.identifier.doi http://dx.doi.org/10.1242/jeb.156646
  • dc.identifier.issn 0022-0949
  • dc.identifier.uri http://hdl.handle.net/10230/34915
  • dc.language.iso eng
  • dc.publisher Company of Biologistsca
  • dc.relation.ispartof J Exp Biol. 2017 Jul 1;220(Pt 13):2452-2475
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/618045
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2011-26208
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/309832
  • dc.rights © Company of Biologists https://doi.org/10.1242/jcs.193821 DOI 10.1242/jeb.156646
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.subject.keyword Feeding
  • dc.subject.keyword Learning and memory
  • dc.subject.keyword Locomotion
  • dc.subject.keyword Navigation
  • dc.subject.keyword Sensory processing
  • dc.title The Ol1mpiad: concordance of behavioural faculties of stage 1 and stage 3 Drosophila larvaeca
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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