C-edge loops of arrestin function as a membrane anchor

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• dc.contributor.author Lally, Ciara C.M.ca
• dc.contributor.author Bauer, Brainca
• dc.contributor.author Selent, Janaca
• dc.contributor.author Sommer, Martha E.ca
• dc.date.accessioned 2017-05-23T08:06:25Z
• dc.date.available 2017-05-23T08:06:25Z
• dc.date.issued 2017
• dc.description.abstract G-protein-coupled receptors are membrane proteins that are regulated by a small family of arrestin proteins. During formation of the arrestin–receptor complex, arrestin first interacts with the phosphorylated receptor C terminus in a pre-complex, which activates arrestin for tight receptor binding. Currently, little is known about the structure of the pre-complex and its transition to a high-affinity complex. Here we present molecular dynamics simulations and site-directed fluorescence experiments on arrestin-1 interactions with rhodopsin, showing that loops within the C-edge of arrestin function as a membrane anchor. Activation of arrestin by receptor-attached phosphates is necessary for C-edge engagement of the membrane, and we show that these interactions are distinct in the pre-complex and high-affinity complex in regard to their conformation and orientation. Our results expand current knowledge of C-edge structure and further illuminate the conformational transitions that occur in arrestin along the pathway to tight receptor binding.
• dc.description.sponsorship This work was supported by grants from the Deutsche Forschungsgemeinschaft (SO1037/1-2 to M.E.S.), the Berlin Institute of Health (Delbrück Fellowship BIH_PRO_314 to M.E.S.) and the Instituto de Salud Carlos III, El Fondo Europeo de Desarrollo Regional (FEDER) (CP12/03139 and PI15/00460 to J.S.). M.E.S. and J.S. participate in the European COST Action CM1207 (GLISTEN).
• dc.format.mimetype application/pdfca
• dc.identifier.citation Lally CCM, Bauer B, Selent J, Sommer ME. C-edge loops of arrestin function as a membrane anchor. Nature Communications. 2017;8:14258. DOI: 10.1038/ncomms14258
• dc.identifier.doi http://dx.doi.org/10.1038/ncomms14258
• dc.identifier.issn 2041-1723
• dc.identifier.uri http://hdl.handle.net/10230/32149
• dc.language.iso eng
• dc.publisher Nature Publishing Groupca
• dc.relation.ispartof Nature Communications. 2017;8:14258
• dc.rights © Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Computational biophysics
• dc.subject.keyword G protein-coupled receptors
• dc.title C-edge loops of arrestin function as a membrane anchorca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion