Deciphering 3'ss selection in the yeast genome reveals an RNA thermosensor that mediates alternative splicing

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• dc.contributor.author Meyer, Markusca
• dc.contributor.author Plass Pórtulas, Mireya, 1982-ca
• dc.contributor.author Pérez Valle, Jorgeca
• dc.contributor.author Eyras Jiménez, Eduardoca
• dc.contributor.author Vilardell, Josepca
• dc.date.accessioned 2012-04-19T10:56:22Z
• dc.date.available 2012-04-19T10:56:22Z
• dc.date.issued 2011ca
• dc.description.abstract Poor understanding of the spliceosomal mechanisms to select intronic 3' ends (3'ss) is a major obstacle to deciphering eukaryotic genomes. Here, we discern the rules for global 3'ss selection in yeast. We show that, in contrast to the uniformity of yeast splicing, the spliceosome uses all available 3'ss within a distance window from the intronic branch site (BS), and that in 70% of all possible 3'ss this is likely to be mediated by pre-mRNA structures. Our results reveal that one of these RNA folds acts as an RNA thermosensor, modulating alternative splicing in response to heat shock by controlling alternate 3'ss availability. Thus, our data point to a deeper role for the pre-mRNA in the control of its own fate, and to a simple mechanism for some alternative splicing.
• dc.description.sponsorship We thank C. Query, J. Valcárcel, and J. Warner for helpful discussions and comments on the manuscript. This research has been supported by the Spanish Ministry of Science (BIO2008-01091 and 363), MC #510183, EURASNET (LSHG-CT-2005-518238), CSIC (200920I195), and Agaur. M. M. is supported in part by a Training of Researchers (FI) fellowship (Agaur) and M. P. by a Carlos III PhD fellowship.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Meyer M, Plass M, Pérez-Valle J, Eyras E, Vilardell J. Deciphering 3'ss selection in the yeast genome reveals an RNA thermosensor that mediates alternative splicing. Mol Cell. 2011;43(6):1033-9. DOI: 10.1016/j.molcel.2011.07.030ca
• dc.identifier.doi http://dx.doi.org/10.1016/j.molcel.2011.07.030
• dc.identifier.issn 1097-2765ca
• dc.identifier.uri http://hdl.handle.net/10230/16375
• dc.language.iso engca
• dc.publisher Elsevierca
• dc.relation.ispartof Molecular Cell. 2011;43(6):1033-9
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2008-01091
• dc.rights © 2011 Elsevier. Published article is available at: http://dx.doi.org/10.1016/j.molcel.2011.07.030ca
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.other Cèl·lules eucariotes -- Aspectes genètics
• dc.subject.other ARN empalmament
• dc.title Deciphering 3'ss selection in the yeast genome reveals an RNA thermosensor that mediates alternative splicingca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/acceptedVersion