A genomic survey of HECT ubiquitin ligases in eukaryotes reveals independent expansions of the HECT system in several lineages

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  • dc.contributor.author Grau Bové, Xavier
  • dc.contributor.author Sebé-Pedrós, Arnau
  • dc.contributor.author Ruiz Trillo, Iñaki
  • dc.date.accessioned 2024-01-23T07:14:52Z
  • dc.date.available 2024-01-23T07:14:52Z
  • dc.date.issued 2013
  • dc.description.abstract The posttranslational modification of proteins by the ubiquitination pathway is an important regulatory mechanism in eukaryotes. To date, however, studies on the evolutionary history of the proteins involved in this pathway have been restricted to E1 and E2 enzymes, whereas E3 studies have been focused mainly in metazoans and plants. To have a wider perspective, here we perform a genomic survey of the HECT family of E3 ubiquitin-protein ligases, an important part of this posttranslational pathway, in genomes from representatives of all major eukaryotic lineages. We classify eukaryotic HECTs and reconstruct, by phylogenetic analysis, the putative repertoire of these proteins in the last eukaryotic common ancestor (LECA). Furthermore, we analyze the diversity and complexity of protein domain architectures of HECTs along the different extant eukaryotic lineages. Our data show that LECA had six different HECTs and that protein expansion and N-terminal domain diversification shaped HECT evolution. Our data reveal that the genomes of animals and unicellular holozoans considerably increased the molecular and functional diversity of their HECT system compared with other eukaryotes. Other eukaryotes, such as the Apusozoa Thecanomas trahens or the Heterokonta Phytophthora infestans, independently expanded their HECT repertoire. In contrast, plant, excavate, rhodophyte, chlorophyte, and fungal genomes have a more limited enzymatic repertoire. Our genomic survey and phylogenetic analysis clarifies the origin and evolution of different HECT families among eukaryotes and provides a useful phylogenetic framework for future evolutionary studies of this regulatory pathway.
  • dc.description.sponsorship The authors thank the Joint Genome Institute and Broad Institute for making data publicly available. This work was supported by an Institució Catalana de Recerca i Estudis Avançats contract, European Research Council starting grant ERC-2007-StG-206883, Ministerio de Economía y Competitividad (MINECO) grant BFU2011-23434 to I.R.-T., and a pregraduate Formación Profesorado Universitario grant from MINECO to A.S.-P.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Grau-Bové X, Sebé-Pedrós A, Ruiz-Trillo I. A genomic survey of HECT ubiquitin ligases in eukaryotes reveals independent expansions of the HECT system in several lineages. Genome Biology and Evolution. 2013 May;5(5):833-47. DOI: 10.1093/gbe/evt052
  • dc.identifier.doi http://dx.doi.org/10.1093/gbe/evt052
  • dc.identifier.issn 1759-6653
  • dc.identifier.uri http://hdl.handle.net/10230/58783
  • dc.language.iso eng
  • dc.publisher Oxford University Press
  • dc.relation.ispartof Genome Biology and Evolution. 2013 May;5(5):833-47
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/206883
  • dc.rights The Author(s) 2013. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by-nc/3.0/
  • dc.subject.keyword Ubiquitination pathway
  • dc.subject.keyword Posttranslational regulation
  • dc.subject.keyword Multicellularity
  • dc.subject.keyword Last common ancestor of eukaryotes
  • dc.subject.keyword Holozoa
  • dc.title A genomic survey of HECT ubiquitin ligases in eukaryotes reveals independent expansions of the HECT system in several lineages
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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