Light modulates the lipidome of the photosynthetic sea slug Elysia timida

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• dc.contributor.author Rey, Felisa
• dc.contributor.author Cartaxana, Paulo
• dc.contributor.author Aveiro, Susana
• dc.contributor.author Greenacre, Michael
• dc.contributor.author Melo, Tânia
• dc.contributor.author Domingues, Pedro
• dc.contributor.author Domingues, M. Rosário
• dc.contributor.author Cruz, Sónia
• dc.date.accessioned 2023-03-13T07:46:16Z
• dc.date.available 2023-03-13T07:46:16Z
• dc.date.issued 2023
• dc.description.abstract Long-term kleptoplasty, the capability to retain functional stolen chloroplasts (kleptoplasts) for several weeks to months, has been shown in a handful of Sacoglossa sea slugs. One of these sea slugs is Elysia timida, endemic to the Mediterranean, which retains functional chloroplasts of the macroalga Acetabularia acetabulum. To understand how light modulates the lipidome of E. timida, sea slug specimens were subjected to two different 4-week light treatments: regular light and quasi-dark conditions. Lipidomic analyses were performed by HILIC-HR-ESI-MS and MS/MS. Quasi-dark conditions caused a reduction in the amount of essential lipids for photosynthetic membranes, such as glycolipids, indicating high level of kleptoplast degradation under sub-optimal light conditions. However, maximum photosynthetic capacities (Fv/Fm) were identical in both light treatments (≈0.75), showing similar kleptoplast functionality and suggesting that older kleptoplasts were targeted for degradation. Although more stable, the phospholipidome showed differences between light treatments: the amount of certain lipid species of phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylglycerol (PG) decreased under quasi-dark conditions, while other lipid species of phosphatidylcholine (PC), PE and lyso-PE (LPE) increased. Quasi-dark conditions promoted a decrease in the relative abundance of polyunsaturated fatty acids. These results suggest a light-driven remodelling of the lipidome according to the functions of the different lipids and highlight the plasticity of polar lipids in the photosynthetic sea slug E. timida.
• dc.description.sponsorship This project has received funding from the European Research Council (ERC) and the European Union's Horizon 2020 research and innovation programme (grant agreement No. 949880). The authors are also thankful to the COST Action EpiLipidNET, CA19105-Pan-European Network in Lipidomics and EpiLipidomics. This work is a contribution of the Marine Lipidomics laboratory. The authors thank the University of Aveiro, Fundação para a Ciência e Tecnologia (FCT), and Ministério da Ciência Tecnologia e Ensino Superior (MCTES) for the financial support to the research units CESAM [UIDB/50017/2020 + UIDP/50017/2020 + LA/P/0094/2020] and LAQV-REQUIMTE (UIDP/50006/2020 + UIDB/50006/2020) through national funds and, where applicable, co-funded by ERDF, within Portugal 2020 Partnership Agreement and Compete 2020, and to the Portuguese Mass Spectrometry Network (RNEM, LISBOA-01-0145-FEDER-402-022125). The authors acknowledge FCT/MCTES for individual funding in the scope of the Individual Call to Scientific Employment Stimulus (CEECIND/00580/2017 to Felisa Rey, CEECIND/01578/2020 to Tânia Melo, 2020.03278.CEECIND to Sónia Cruz and CEECIND/01434/2018 to Paulo Cartaxana). Thanks are due to Dr. Gonçalo Calado, Dr. José Templado and Dr. Marta Calvo for help in the collection of E. timida and A. acetabulum.
• dc.format.mimetype application/pdf
• dc.identifier.citation Rey F, Cartaxana P, Aveiro S, Greenacre M, Melo T, Domingues P, et al. Light modulates the lipidome of the photosynthetic sea slug Elysia timida. Biochim Biophys Acta. 2023 Feb;1868(2):159249. DOI: 10.1016/j.bbalip.2022.159249
• dc.identifier.doi http://dx.doi.org/10.1016/j.bbalip.2022.159249
• dc.identifier.issn 1388-1981
• dc.identifier.uri http://hdl.handle.net/10230/56183
• dc.language.iso eng
• dc.publisher Elsevier
• dc.relation.ispartof Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 2023 Feb;1868(2):159249
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/949880
• dc.rights © 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
• dc.subject.keyword Fatty acids
• dc.subject.keyword Kleptoplasty
• dc.subject.keyword Lipidomics
• dc.subject.keyword Photosynthesis
• dc.subject.keyword Polar lipids
• dc.subject.keyword Sacoglossa
• dc.title Light modulates the lipidome of the photosynthetic sea slug Elysia timida
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion