Tumor induction by an endogenous K-ras oncogene is highly dependent on cellular context

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• dc.contributor.author Guerra, Carmenca
• dc.contributor.author Mijimolle, Nievesca
• dc.contributor.author Dhawahir, Almaca
• dc.contributor.author Dubus, Pierreca
• dc.contributor.author Barradas, Martaca
• dc.contributor.author Serrano, Manuelca
• dc.contributor.author Campuzano Uceda, María Victoriaca
• dc.contributor.author Barbacid, Marianoca
• dc.date.accessioned 2016-02-18T14:36:34Z
• dc.date.available 2016-02-18T14:36:34Z
• dc.date.issued 2003
• dc.description.abstract We have targeted a K-ras allele in mouse embryonic stem (ES) cells to express a K-Ras(V12) oncoprotein along with a marker protein (beta-geo) from a single bicistronic transcript. Expression of this oncogenic allele requires removal of a knocked in STOP transcriptional cassette by Cre recombinase. Primary mouse embryonic fibroblasts expressing this K-ras(V12) allele do not undergo proliferative senescence and proliferate as immortal cells. In mice, expression of K-ras(V12) throughout the body fails to induce unscheduled proliferation or other growth abnormalities for up to eight months. Only a percentage of K-ras(V12)-expressing lung bronchiolo-alveolar cells undergo malignant transformation leading to the formation of multiple adenomas and adenocarcinomas. These results indicate that neoplastic growth induced by an endogenous K-ras oncogene depends upon cellular contextca
• dc.description.sponsorship This work was supported by grants from the V Framework Programme of the EU (to M.B. and M.S.), Ministerio de Ciencia y Tecnologı́a (to M.B.), Ministerio de Sanidad y Consumo and Comunidad Autónoma de Madrid (to C.G.), and Ligue contre le Cancer (Comité de Dordogne) and INSERM (to P.D.). N.M. was supported by a Beca de Formación para la Investigación (BEFI, Fondo de Investigación Sanitaria) and A.D. by a fellowship from the Programa de Formación de Personal Investigador (FPI, Ministerio de Ciencia y Tecnología)
• dc.format.mimetype application/pdfca
• dc.identifier.citation Guerra C, Mijimolle N, Dhawahir A, Dubus P, Barradas M, Serrano M et al. Tumor induction by an endogenous K-ras oncogene is highly dependent on cellular context. Cancer cell. 2003; 4(2): 111-120. DOI 10.1016/S1535-6108(03)00191-0ca
• dc.identifier.doi http://dx.doi.org/10.1016/S1535-6108(03)00191-0
• dc.identifier.issn 1535-6108
• dc.identifier.uri http://hdl.handle.net/10230/25890
• dc.language.iso engca
• dc.publisher Elsevierca
• dc.relation.ispartof Cancer cell. 2003; 4(2): 111-120
• dc.rights © Elsevier. This is the published version of an article http://dx.doi.org/10.1016/S1535-6108(03)00191-0 that appeared in the journal Cancer cell. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-licenseca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.subject.other Proteïnesca
• dc.subject.other Cèl·lules -- Divisióca
• dc.title Tumor induction by an endogenous K-ras oncogene is highly dependent on cellular contextca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca