Role of C5aR1 and C5L2 receptors in ischemia-reperfusion injury

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• dc.contributor.author Arias Cabrales, Carlos Enrique
• dc.contributor.author Rodriguez García, Eva
• dc.contributor.author Gimeno Beltran, Javier
• dc.contributor.author Benito, David
• dc.contributor.author Pérez-Sáez, María José
• dc.contributor.author Redondo Pachón, María Dolores
• dc.contributor.author Buxeda, Anna
• dc.contributor.author Burballa Tàrrega, Carla, 1988-
• dc.contributor.author Crespo Barrio, Marta
• dc.contributor.author Riera, Marta
• dc.contributor.author Pascual Santos, Julio
• dc.date.accessioned 2021-09-29T06:38:26Z
• dc.date.available 2021-09-29T06:38:26Z
• dc.date.issued 2021
• dc.description.abstract The role of C5a receptors (C5aR1 and C5L2) in renal ischemia-reperfusion injury (IRI) is uncertain. We generated an in vitro model of hypoxia/reoxygenation with human proximal tubule epithelial cells to mimic some IRI events. C5aR1, membrane attack complex (MAC) and factor H (FH) deposits were evaluated with immunofluorescence. Quantitative polymerase chain reaction evaluated the expression of C5aR1, C5L2 genes as well as genes related to tubular injury, inflammation, and profibrotic pathways. Additionally, C5aR1 and C5L2 deposits were evaluated in kidney graft biopsies (KB) from transplant patients with delayed graft function (DGF, n = 12) and compared with a control group (n = 8). We observed higher immunofluorescence expression of C5aR1, MAC and FH as higher expression of genes related to tubular injury, inflammatory and profibrotic pathways and of C5aR1 in the hypoxic cells; whereas, C5L2 gene expression was unaffected by the hypoxic stimulus. Regarding KB, C5aR1 was detected in the apical and basal membrane of tubular epithelial cells, whereas C5L2 deposits were observed in endothelial cells of peritubular capillaries (PTC). DGF-KB showed more frequently diffuse C5aR1 staining and C5L2 compared to controls. In conclusion, C5aR1 expression is increased by hypoxia and IRI, both in vitro and in human biopsies with an acute injury. C5L2 expression in PTC could be related to endothelial cell damage during IRI.
• dc.format.mimetype application/pdf
• dc.identifier.citation Arias-Cabrales C, Rodriguez-Garcia E, Gimeno J, Benito D, Pérez-Sáez MJ, Redondo-Pachón D, Buxeda A, Burballa C, Crespo M, Riera M, Pascual J. Role of C5aR1 and C5L2 receptors in ischemia-reperfusion injury. J Clin Med. 2021;10(5):974. DOI: 10.3390/jcm10050974
• dc.identifier.doi http://dx.doi.org/10.3390/jcm10050974
• dc.identifier.issn 2077-0383
• dc.identifier.uri http://hdl.handle.net/10230/48527
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof J Clin Med. 2021;10(5):974
• dc.rights © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword C5a receptors
• dc.subject.keyword Complement system
• dc.subject.keyword Delayed graft function
• dc.subject.keyword Ischemia-reperfusion injury
• dc.subject.keyword Kidney transplant
• dc.title Role of C5aR1 and C5L2 receptors in ischemia-reperfusion injury
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion