Polyploidy as a strategy to increase taxane production in yew cell cultures: Obtaining and characterizing a Taxus baccata tetraploid cell line
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- dc.contributor.author Escrich Montañana, Ainoa
- dc.contributor.author Hidalgo Martínez, Diego
- dc.contributor.author Bonfill Baldrich, Ma. Mercedes
- dc.contributor.author Palazón Barandela, Javier
- dc.contributor.author Sánchez Muñoz, Raúl, 1990-
- dc.contributor.author Moyano Claramunt, Elisabet
- dc.date.accessioned 2024-03-26T07:25:40Z
- dc.date.available 2024-03-26T07:25:40Z
- dc.date.issued 2023
- dc.description.abstract Novel approaches to optimize the production of plant specialized metabolites are crucial to reach maximum productivity of plant biofactories. Plant polyploidization frequently enhances protein synthesis and thereby increases the biosynthesis of specialized metabolites. Paclitaxel is a valuable anticancer agent scarcely produced in nature. Therefore, plant biofactories represent a sustainable alternative source of this compound and related taxanes. With the aim of improving the productivity of Taxus spp. cell cultures, we induced polyploidy in vitro by treating immature embryos of Taxus baccata with colchicine. To obtain the polyploid cell lines, calli were induced from T. baccata plantlets previously treated with colchicine and ploidy levels were accurately identified using flow cytometry. In terms of cell morphology, tetraploid cells were about 3-fold bigger than the diploid cells. The expression of taxane pathway genes was higher in the tetraploid cell line compared to the diploid cells. Moreover, taxane production was 6.2-fold higher and the production peak was achieved 8 days earlier than in the diploid cell line, indicating a higher productivity. The obtained tetraploid cell line proved to be highly productive, constituting a step forward towards the development of a bio-sustainable production system for this chemotherapeutic drug.
- dc.description.sponsorship This work was supported by the Spanish Ministry of Science and Innovation, with project number PID2020–113438RB-I00/AEI/10.13039/501100011033. Ainoa Escrich was supported by a fellowship from the University Pompeu Fabra.
- dc.format.mimetype application/pdf
- dc.identifier.citation Escrich A, Hidalgo D, Bonfill M, Palazon J, Sanchez-Muñoz R, Moyano E. Polyploidy as a strategy to increase taxane production in yew cell cultures: Obtaining and characterizing a Taxus baccata tetraploid cell line. Plant Sci. 2023 Sep;334:111776. DOI: 10.1016/j.plantsci.2023.111776
- dc.identifier.doi http://dx.doi.org/10.1016/j.plantsci.2023.111776
- dc.identifier.issn 0168-9452
- dc.identifier.uri http://hdl.handle.net/10230/59578
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof Plant Sci. 2023 Sep;334:111776
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020–113438RB-I00
- dc.rights © 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Cell cultures
- dc.subject.keyword Colchicine
- dc.subject.keyword Paclitaxel
- dc.subject.keyword Polyploidy
- dc.subject.keyword Taxane productivity
- dc.subject.keyword Taxus spp
- dc.title Polyploidy as a strategy to increase taxane production in yew cell cultures: Obtaining and characterizing a Taxus baccata tetraploid cell line
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion