Hair cell identity establishes labeled lines of directional mechanosensation

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• dc.contributor.author Lozano Ortega, Marta
• dc.contributor.author Valera, Gema
• dc.contributor.author Xiao, Yan
• dc.contributor.author Faucherre, Adèle
• dc.contributor.author López-Schier, Hernán
• dc.date.accessioned 2019-12-04T08:32:59Z
• dc.date.available 2019-12-04T08:32:59Z
• dc.date.issued 2018
• dc.description.abstract Directional mechanoreception by hair cells is transmitted to the brain via afferent neurons to enable postural control and rheotaxis. Neuronal tuning to individual directions of mechanical flow occurs when each peripheral axon selectively synapses with multiple hair cells of identical planar polarization. How such mechanosensory labeled lines are established and maintained remains unsolved. Here, we use the zebrafish lateral line to reveal that asymmetric activity of the transcription factor Emx2 diversifies hair cell identity to instruct polarity-selective synaptogenesis. Unexpectedly, presynaptic scaffolds and coherent hair cell orientation are dispensable for synaptic selectivity, indicating that epithelial planar polarity and synaptic partner matching are separable. Moreover, regenerating axons recapitulate synapses with hair cells according to Emx2 expression but not global orientation. Our results identify a simple cellular algorithm that solves the selectivity task even in the presence of noise generated by the frequent receptor cell turnover. They also suggest that coupling connectivity patterns to cellular identity rather than polarity relaxes developmental and evolutionary constraints to innervation of organs with differing orientation.
• dc.description.sponsorship Intra-European Marie Curie Fellowship (grant number). Received by AF. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. European Research Council (grant number 205095). Received by HLS. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
• dc.format.mimetype application/pdf
• dc.identifier.citation Lozano-Ortega M, Valera G, Xiao Y, Faucherre A, López-Schier H. Hair cell identity establishes labeled lines of directional mechanosensation. PLoS Biol. 2018; 16(7):e2004404. DOI 10.1371/journal.pbio.2004404
• dc.identifier.doi http://dx.doi.org/10.1371/journal.pbio.2004404
• dc.identifier.issn 1544-9173
• dc.identifier.uri http://hdl.handle.net/10230/43078
• dc.language.iso eng
• dc.publisher Public Library of Science (PLoS)
• dc.relation.ispartof PLoS Biol. 2018; 16(7):e2004404
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/205095
• dc.rights © 2018 Lozano-Ortega et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Axons
• dc.subject.keyword Synapses
• dc.subject.keyword Planar cell polarity
• dc.subject.keyword Cell polarity
• dc.subject.keyword Green fluorescent protein
• dc.subject.keyword Zebrafish
• dc.subject.keyword Neuronal tuning
• dc.subject.keyword Afferent neurons
• dc.title Hair cell identity establishes labeled lines of directional mechanosensation
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion