A DNA methylation fingerprint of 1628 human samples

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  • dc.contributor.author Pérez Jurado, Luis Albertoca
  • dc.contributor.author Fernández, Agustín F.ca
  • dc.contributor.author Esteller, Manelca
  • dc.date.accessioned 2015-06-09T08:10:59Z
  • dc.date.available 2015-06-09T08:10:59Z
  • dc.date.issued 2012ca
  • dc.description.abstract Most of the studies characterizing DNA methylation patterns have been restricted to particular genomic loci in a limited number of human samples and pathological conditions. Herein, we present a compromise between an extremely comprehensive study of a human sample population with an intermediate level of resolution of CpGs at the genomic level. We obtained a DNA methylation fingerprint of 1628 human samples in which we interrogated 1505 CpG sites. The DNA methylation patterns revealed show this epigenetic mark to be critical in tissue-type definition and stemness, particularly around transcription start sites that are not within a CpG island. For disease, the generated DNA methylation fingerprints show that, during tumorigenesis, human cancer cells underwent a progressive gain of promoter CpG-island hypermethylation and a loss of CpG methylation in non-CpG-island promoters. Although transformed cells are those in which DNA methylation disruption is more obvious, we observed that other common human diseases, such as neurological and autoimmune disorders, had their own distinct DNA methylation profiles. Most importantly, we provide proof of principle that the DNA methylation fingerprints obtained might be useful for translational purposes by showing that we are able to identify the tumor type origin of cancers of unknown primary origin (CUPs). Thus, the DNA methylation patterns identified across the largest spectrum of samples, tissues, and diseases reported to date constitute a baseline for developing higher-resolution DNA methylation maps and provide important clues concerning the contribution of CpG methylation to tissue identity and its changes in the most prevalent human diseases.en
  • dc.description.sponsorship This work was supported by European Grants CANCER DIP HEALTH-F2-2007-200620, LSHG-CT-2006-018739-ESTOOLS, LSHC-CT-2006-037297- MCSCs, the Dr. Josef Steiner Cancer Research Foundation Award, the Fondo de Investigaciones Sanitarias Grant PI08-1345, Consolider Grant MEC09-05, the Spanish Association Against Cancer (AECC), Spanish Ministry of Education and Science (SAF2009-07319), the Lilly Foundation Biomedical ResearchAward, Fundació Cellex, and the Health Department of the Catalan Government (Generalitat de Catalunya). R.S. is supported by BMBF and Deutsche Krebshilfe. J.B. is supported in part by the University College London Cancer Institute Experimental Cancer Medicine Centre and the University College London Hospitals and University College London Comprehensive Biomedical Research Centre. M.E. is an Institució Catalana de Recerca i Estudis Avançats (ICREA) Research Professoren
  • dc.format.mimetype application/pdfca
  • dc.identifier.citation Fernández AF, Assenov Y, Martín-Subero JI, Balint B, Siebert R, Taniguchi H et al. A DNA methylation fingerprint of 1628 human samples. Genome Research. 2012; 22: 407-419. DOI 10.1101/gr.119867.110ca
  • dc.identifier.doi http://dx.doi.org/10.1101/gr.119867.110
  • dc.identifier.issn 1088-9051ca
  • dc.identifier.uri http://hdl.handle.net/10230/23772
  • dc.language.iso engca
  • dc.publisher Cold Spring Harbor Laboratory Press (CSHL Press)ca
  • dc.relation.ispartof Genome Research. 2012; 22: 407-419
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/200620
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/018739
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/037297
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2009-07319
  • dc.rights Copyright © 2012 by Cold Spring Harbor Laboratory Press
  • dc.rights.accessRights info:eu-repo/semantics/openAccessca
  • dc.subject.other Genètica humana -- Variacióca
  • dc.title A DNA methylation fingerprint of 1628 human samplesen
  • dc.type info:eu-repo/semantics/articleca
  • dc.type.version info:eu-repo/semantics/publishedVersionca

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