The peroxiredoxin Tpx1 is essential as a H202-scavenger during aerobic growth in fission yeast

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• dc.contributor.author Jara, Mónicaca
• dc.contributor.author Vivancos Prellezo, Anaca
• dc.contributor.author Calvo, Isabel A.ca
• dc.contributor.author Moldón Vara, Albertoca
• dc.contributor.author Sansó Martínez, Miriam, 1979-ca
• dc.contributor.author Hidalgo Hernando, Elenaca
• dc.date.accessioned 2012-04-17T12:11:01Z
• dc.date.available 2012-04-17T12:11:01Z
• dc.date.issued 2007ca
• dc.description.abstract Peroxiredoxins are known to interact with hydrogen peroxide (H2O2) and to participate in oxidant scavenging, redox signal transduction, and heat-shock responses. The two-cysteine peroxiredoxin Tpx1 of Schizosaccharomyces pombe has been characterized as the H2O2 sensor that transduces the redox signal to the transcription factor Pap1. Here, we show that Tpx1 is essential for aerobic, but not anaerobic, growth. We demonstrate that Tpx1 has an exquisite sensitivity for its substrate, which explains its participation in maintaining low steady-state levels of H2O2. We also show in vitro and in vivo that inactivation of Tpx1 by oxidation of its catalytic cysteine to a sulfinic acid is always preceded by a sulfinic acid form in a covalently linked dimer, which may be important for understanding the kinetics of Tpx1 inactivation. Furthermore, we provide evidence that a strain expressing Tpx1.C169S, lacking the resolving cysteine, can sustain aerobic growth, and we show that small reductants can modulate the activity of the mutant protein in vitro, probably by supplying a thiol group to substitute for cysteine 169.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Jara M, Vivancos AP, Calvo IA, Moldón A, Sansó M, Hidalgo E. The peroxirredoxin Tpx1 is essential as a H202-scavenger during aerobic growth in fission yeast. Mol. Biol. Cell. 2007;18(6):2288-95. DOI: 10.1091/mbc.E06-11-1039ca
• dc.identifier.doi http://dx.doi.org/10.1091/mbc.E06-11-1039
• dc.identifier.issn 1939-4586ca
• dc.identifier.uri http://hdl.handle.net/10230/16369
• dc.language.iso engca
• dc.publisher American Society for Cell Biologyca
• dc.relation.ispartof Molecular Biology of the Cell. 2007;18(6):2288-95
• dc.rights © 2007. The American Society for Cell Biology. Document publicat disponible a: http://www.molbiolcell.org/content/18/6/2288.full. Aquest document està subjecte a una llicència Creative Commonsca
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.other Peroxidasa
• dc.subject.other Estrès oxidatiu
• dc.title The peroxiredoxin Tpx1 is essential as a H202-scavenger during aerobic growth in fission yeastca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersion