Genome-wide screen of genes required for caffeine tolerance in fission yeast

Calvo, Isabel A.; Gabrielli, Natalia, 1978-; Iglesias Baena, Iván; García Santamarina, Sarela, 1978-; Hoe, Kwang-Lae; Kim, Dong Uk; Sansó Martínez, Miriam, 1979-; Zuin, Alice, 1978-; Pérez, Pilar; Ayté del Olmo, José; Hidalgo Hernando, Elena

Genome-wide screen of genes required for caffeine tolerance in fission yeast

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dc.contributor.author Calvo, Isabel A.ca
dc.contributor.author Gabrielli, Natalia, 1978-ca
dc.contributor.author Iglesias Baena, Ivánca
dc.contributor.author García Santamarina, Sarela, 1978-ca
dc.contributor.author Hoe, Kwang-Laeca
dc.contributor.author Kim, Dong Ukca
dc.contributor.author Sansó Martínez, Miriam, 1979-ca
dc.contributor.author Zuin, Alice, 1978-ca
dc.contributor.author Pérez, Pilarca
dc.contributor.author Ayté del Olmo, Joséca
dc.contributor.author Hidalgo Hernando, Elenaca
dc.date.accessioned 2012-04-16T16:05:36Z
dc.date.available 2012-04-16T16:05:36Z
dc.date.issued 2009ca
dc.description.abstract Background: An excess of caffeine is cytotoxic to all eukaryotic cell types. We aim to study how cells become tolerant to a/ntoxic dose of this drug, and the relationship between caffeine and oxidative stress pathways./nMethodology/Principal Findings: We searched for Schizosaccharomyces pombe mutants with inhibited growth on caffeinecontaining/nplates. We screened a collection of 2,700 haploid mutant cells, of which 98 were sensitive to caffeine. The genes mutated in these sensitive clones were involved in a number of cellular roles including the H2O2-induced Pap1 and Sty1 stress pathways, the integrity and calcineurin pathways, cell morphology and chromatin remodeling. We have investigated the role of the oxidative stress pathways in sensing and promoting survival to caffeine. The Pap1 and the Sty1 pathways are both required for normal tolerance to caffeine, but only the Sty1 pathway is activated by the drug. Cells lacking Pap1 are/nsensitive to caffeine due to the decreased expression of the efflux pump Hba2. Indeed, ?hba2 cells are sensitive to caffeine, and constitutive activation of the Pap1 pathway enhances resistance to caffeine in an Hba2-dependent manner. Conclusions/Significance: With our caffeine-sensitive, genome-wide screen of an S. pombe deletion collection, we have/ndemonstrated the importance of some oxidative stress pathway components on wild-type tolerance to the drug.
dc.format.mimetype application/pdfca
dc.identifier.citation Calvo IA, Gabrielli N, Iglesias-Baena I, García-Santamarina S, Hoe K-L, Kim DU, Sansó M, Zuin A, Pérez P, Ayté J, Hidalgo E. Genome-Wide Screen of Genes Required for Caffeine Tolerance in Fission Yeast. PLoS ONE. 2009;4(8):e6619. DOI: 10.1371/journal.pone.0006619ca
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0006619
dc.identifier.issn 1932-6203ca
dc.identifier.uri http://hdl.handle.net/10230/16364
dc.language.iso engca
dc.publisher Public Library of Science (PLoS)ca
dc.relation.ispartof PLoS ONE. 2009;4(8):e6619
dc.rights © 2009 Calvo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ca
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by/2.5/
dc.subject.other Cafeïna
dc.subject.other Cèl·lules eucariotes -- Aspectes genètics
dc.title Genome-wide screen of genes required for caffeine tolerance in fission yeastca
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/publishedVersion

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