The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms

Jungfleisch, Jennifer, 1986-; Chowdhury, Ashis; Alves-Rodrigues, Isabel; Tharun, Sundaresan; Díez Antón, Juana, 1962-

The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms

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dc.contributor.author Jungfleisch, Jennifer, 1986-ca
dc.contributor.author Chowdhury, Ashisca
dc.contributor.author Alves-Rodrigues, Isabelca
dc.contributor.author Tharun, Sundaresanca
dc.contributor.author Díez Antón, Juana, 1962-ca
dc.date.accessioned 2016-02-03T16:26:47Z
dc.date.available 2016-09-01T02:00:05Z
dc.date.issued 2015
dc.description.abstract The Lsm1-7-Pat1 complex binds to the 3' end of cellular mRNAs and promotes 3' end protection and 5'-3' decay. Interestingly, this complex also specifically binds to cis-acting regulatory sequences of viral positive-strand RNA genomes promoting their translation and subsequent recruitment from translation to replication. Yet, how the Lsm1-7-Pat1 complex regulates these two processes remains elusive. Here, we show that Lsm1-7-Pat1 complex acts differentially in these processes. By using a collection of well-characterized lsm1 mutant alleles and a system that allows the replication of Brome mosaic virus (BMV) in yeast we show that the Lsm1-7-Pat1 complex integrity is essential for both, translation and recruitment. However, the intrinsic RNA-binding ability of the complex is only required for translation. Consistent with an RNA-binding-independent function of the Lsm1-7-Pat1 complex on BMV RNA recruitment, we show that the BMV 1a protein, the sole viral protein required for recruitment, interacts with this complex in an RNA-independent manner. Together, these results support a model wherein Lsm1-7-Pat1 complex binds consecutively to BMV RNA regulatory sequences and the 1a protein to promote viral RNA translation and later recruitment out of the host translation machinery to the viral replication complexes.ca
dc.description.sponsorship This work was supported by a grant from the Spanish Ministerio de Ciencia e Innovación (BFU2013-44629-R) and USUHS intramural grant to S.T. J.J. was supported by grant 2012FI_B00574 from the Generalitat de Catalunya.
dc.format.mimetype application/pdfca
dc.identifier.citation Jungfleisch J, Chowdhury A, Alves-Rodrigues I, Tharun S, Díez J. The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanisms. RNA. 2015;21(8):1469-79. DOI: 10.1261/rna.052209.115ca
dc.identifier.doi http://dx.doi.org/10.1261/rna.052209.115
dc.identifier.issn 1355-8382
dc.identifier.uri http://hdl.handle.net/10230/25729
dc.language.iso engca
dc.publisher Cold Spring Harbor Laboratory Pressca
dc.relation.ispartof RNA. 2015;21(8):1469-79
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2013-44629-R
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/ca
dc.subject.keyword Lsm1-7
dc.subject.keyword Pat1
dc.subject.keyword Lsm1-7-Pat1 complex
dc.subject.keyword Decapping activators
dc.subject.keyword Viral RNA
dc.subject.keyword BMV
dc.subject.keyword RNA virus
dc.subject.keyword Lsm proteins
dc.subject.keyword Translation
dc.subject.keyword Replication
dc.subject.other Saccharomyces cerevisiae -- Metabolismeca
dc.subject.other RNA missatgerca
dc.title The Lsm1-7-Pat1 complex promotes viral RNA translation and replication by differential mechanismsca
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/publishedVersionca

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