Monitoring B cell subsets and alloreactivity in kidney transplantation

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• dc.contributor.author Crespo Barrio, Martaca
• dc.contributor.author Heidt, Sebastiaanca
• dc.contributor.author Redondo Pachón, María Doloresca
• dc.contributor.author Pascual Santos, Julioca
• dc.date.accessioned 2015-06-05T09:57:17Z
• dc.date.available 2016-04-30T02:00:03Z
• dc.date.issued 2015
• dc.description.abstract B cells are the precursors of antibody producing plasma cells that can give rise to the formation of donor-specific antibodies. However, recent data suggest that besides their role in antibody production, B cells participate in antibody-independent responses, potentially leading to allograft rejection or allograft tolerance. The presence of CD20+ B cells in kidney graft biopsies has been shown during severe acute rejection episodes and during chronic rejection. Furthermore, operationally tolerant kidney transplant recipients showed a clear B cell dominated fingerprint of tolerance. Several techniques exist to study B cells on different levels. Numerous classification schemes allow for the distinction of many different B cell subsets using flow cytometry. Regardless, data on B cell subsets during stable graft function, rejection or tolerance remain scarce. To obtain a complete picture of the role of B cells during transplantation, antigen specific B cell assays may be required. Therefore, techniques have now been developed that allow for studying the specificity and frequency of HLA specific B cells. Here, we present an overview of the existent assays, panels and techniques intended to characterize peripheral B cells, and the currently available HLA specific B cell functional assays that may allow for monitoring the humoral alloimmune response in transplant recipients.ca
• dc.description.sponsorship This study was performed with funding from the projects PI10/01370, PI13/00598 SpanishMinistry of Health ISCIII FIS-FEDER, Marato TV3 137/C/2012 and RedinRen RD12/0021/0024.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Crespo M, Heidt S, Redondo D, Pascual J. Monitoring B cell subsets and alloreactivity in kidney transplantation. Transplant Rev (Orlando). 2015 Apr;29(2):45-52. doi: 10.1016/j.trre.2015.02.001.ca
• dc.identifier.doi http://dx.doi.org/10.1016/j.trre.2015.02.001
• dc.identifier.issn 0955-470X
• dc.identifier.uri http://hdl.handle.net/10230/23747
• dc.language.iso engca
• dc.publisher Elsevierca
• dc.relation.ispartof Transplantation Reviews. 2015 Apr;29(2):45-52
• dc.rights © 2015 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://dx.doi.org/10.1016/j.trre.2015.02.001ca
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.other Ronyons -- Trasplantacióca
• dc.title Monitoring B cell subsets and alloreactivity in kidney transplantationca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/acceptedVersionca