Immunogenicity of foot-and-mouth disease virus dendrimer peptides: need for a T-cell epitope and ability to elicit heterotypic responses

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  • dc.contributor.author Cañas Arranz, Rodrigo
  • dc.contributor.author de León, Patricia
  • dc.contributor.author Defaus, Sira
  • dc.contributor.author Torres, Elisa
  • dc.contributor.author Forner, Mar, 1980-
  • dc.contributor.author Bustos, María J.
  • dc.contributor.author Andreu Martínez, David
  • dc.contributor.author Blanco, Esther
  • dc.contributor.author Sobrino, Francisco
  • dc.date.accessioned 2021-10-15T06:17:59Z
  • dc.date.available 2021-10-15T06:17:59Z
  • dc.date.issued 2021
  • dc.description.abstract An approach based on a dendrimer display of B- and T-cell epitopes relevant for antibody induction has been shown to be effective as a foot-and-mouth disease (FMD) vaccine. B2T dendrimers combining two copies of the major FMD virus (FMDV) type O B-cell epitope (capsid proteinVP1 (140-158)) covalently linked to a heterotypic T-cell epitope from non-structural protein 3A (21-35), henceforth B2T-3A, has previously been shown to elicit high neutralizing antibody (nAb) titers and IFN-γ-producing cells in both mice and pigs. Here, we provide evidence that the B- and T-cell epitopes need to be tethered to a single molecular platform for successful T-cell help, leading to efficient nAb induction in mice. In addition, mice immunized with a non-covalent mixture of B2T-3A dendrimers containing the B-cell epitopes of FMDV types O and C induced similarly high nAb levels against both serotypes, opening the way for a multivalent vaccine platform against a variety of serologically different FMDVs. These findings are relevant for the design of vaccine strategies based on B- and T-cell epitope combinations.
  • dc.description.sponsorship This research was funded by the Spanish Ministry of Science, Innovation and Universities (grants AGL2014-48923-C2 and AGL2017-84097-C2-2-R (to D.A. and F.S), and PID2019-107145RB-100 (to E.B.)), as well as by Comunidad de Madrid co-financed ECFEDER funds (S2013/ABI-350 2906-PLATESA and P2018/BAA-4370 to F.S. and E.B.), and by Generalitat de Catalunya (2009SGR492 to D.A.). Work at Centro de Biología Molecular “Severo Ochoa” and at UPF was supported by the Fundación Ramón Areces and by the Maria de Maeztu Program of the Spanish Ministry of Science, Innovation and Universities, respectively. M.F. and R.C.-A. were holders of a PhD fellowship from the Spanish Ministry of Science, Innovation and Universities.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Cañas-Arranz R, de León P, Defaus S, Torres E, Forner M, Bustos MJ, Andreu D, Blanco E, Sobrino F. Immunogenicity of foot-and-mouth disease virus dendrimer peptides: need for a T-cell epitope and ability to elicit heterotypic responses. Molecules. 2021;26(16):4714. DOI: 10.3390/molecules26164714
  • dc.identifier.doi http://dx.doi.org/10.3390/molecules26164714
  • dc.identifier.issn 1420-3049
  • dc.identifier.uri http://hdl.handle.net/10230/48664
  • dc.language.iso eng
  • dc.publisher MDPI
  • dc.relation.ispartof Molecules. 2021;26(16):4714
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/AGL2014-48923-C2
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/AGL2017-84097-C2-2-R
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2019-107145RB-100
  • dc.rights © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword FMDV
  • dc.subject.keyword Dendrimer
  • dc.subject.keyword Peptide vaccine
  • dc.title Immunogenicity of foot-and-mouth disease virus dendrimer peptides: need for a T-cell epitope and ability to elicit heterotypic responses
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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