Direct cloning of double-stranded RNAs from RNase protection analysis reveals processing patterns of C/D box snoRNAs and provides evidence for widespread antisense transcript expression
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- dc.contributor.author Shen, Manlica
- dc.contributor.author Eyras Jiménez, Eduardoca
- dc.contributor.author Wu, Jieca
- dc.contributor.author Josiah, Sereneca
- dc.contributor.author Rederstorff, Mathieuca
- dc.contributor.author Zhang, Michael Qca
- dc.contributor.author Stamm, Stefanca
- dc.date.accessioned 2015-04-07T09:36:06Z
- dc.date.available 2015-04-07T09:36:06Z
- dc.date.issued 2011ca
- dc.description.abstract We describe a new method that allows cloning of double-stranded RNAs (dsRNAs) that are generated in RNase protection experiments. We demonstrate that the mouse C/D box snoRNA MBII-85 (SNORD116) is processed into at least five shorter RNAs using processing sites near known functional elements of C/D box snoRNAs. Surprisingly, the majority of cloned RNAs from RNase protection experiments were derived from endogenous cellular RNA, indicating widespread antisense expression. The cloned dsRNAs could be mapped to genome areas that show RNA expression on both DNA strands and partially overlapped with experimentally determined argonaute-binding sites. The data suggest a conserved processing pattern for some C/D box snoRNAs and abundant expression of longer, non-coding RNAs in the cell that can potentially form dsRNAs.en
- dc.description.sponsorship National Institutes of Health (RO1 GM083187 to S.S.; NIH GM074688 to M.Z.); the Prader-Willi Research Foundation USA and Shire Human Genetic Therapies; European Comission project EURASNET-LSHG-CT- 2005-518238 (to S.S. and E.E.); Spanish Ministry of Science grant BIO2008-01091 (to E.E.). Funding for open access charge: National Institutes of Health (RO1 GM083187)en
- dc.format.mimetype application/pdfca
- dc.identifier.citation Shen M, Eyras E, Wu J, Khanna A, Josiah S, Rederstorff M, Zhang MQ, Stamm S. Direct cloning of double-stranded RNAs from RNase protection analysis reveals processing patterns of C/D box snoRNAs and provides evidence for widespread antisense transcript expression. Nucleic Acids Research. 2011;39(22):9720-30. DOI: 10.1093/nar/gkr684ca
- dc.identifier.doi http://dx.doi.org/10.1093/nar/gkr684
- dc.identifier.issn 0305-1048ca
- dc.identifier.uri http://hdl.handle.net/10230/23340
- dc.language.iso engca
- dc.publisher Oxford University Pressca
- dc.relation.ispartof Nucleic Acids Research. 2011;39(22):9720-30
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP6/518238
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2008-01091
- dc.rights © Fernandez N, Fernandez-Miragall O, Ramajo J, Garcia-Sacristan A, Bellora N, Eyras E, Briones C, Martinez-Salas E [2011]. Published by Oxford University Press. This is an Open Access article distributed under the terms of a Creative Commons Attribution Licenseca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri http://creativecommons.org/licenses/by-nc/3.0
- dc.subject.other RNAca
- dc.subject.other Seqüència de nucleòtidsca
- dc.subject.other Clonatge molecularca
- dc.subject.other Proteïnes -- Metabolismeca
- dc.title Direct cloning of double-stranded RNAs from RNase protection analysis reveals processing patterns of C/D box snoRNAs and provides evidence for widespread antisense transcript expressionen
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca