Bacterial antisense RNAs are mainly the product of transcriptional noise
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Lloréns Rico, Verónica, 1989-ca
- dc.contributor.author Cano, Jaimeca
- dc.contributor.author Kamminga, Tjerkoca
- dc.contributor.author Gil, Rosarioca
- dc.contributor.author Latorre, Amparoca
- dc.contributor.author Chen, Wei-Huaca
- dc.contributor.author Bork, Peerca
- dc.contributor.author Glass, John I.ca
- dc.contributor.author Serrano Pubull, Luis, 1982-ca
- dc.contributor.author Lluch-Senar, Maria 1982-ca
- dc.date.accessioned 2017-04-26T10:07:31Z
- dc.date.available 2017-04-26T10:07:31Z
- dc.date.issued 2016
- dc.description.abstract cis-Encoded antisense RNAs (asRNAs) are widespread along bacterial transcriptomes. However, the role of most of these RNAs remains unknown, and there is an ongoing discussion as to what extent these transcripts are the result of transcriptional noise. We show, by comparative transcriptomics of 20 bacterial species and one chloroplast, that the number of asRNAs is exponentially dependent on the genomic AT content and that expression of asRNA at low levels exerts little impact in terms of energy consumption. A transcription model simulating mRNA and asRNA production indicates that the asRNA regulatory effect is only observed above certain expression thresholds, substantially higher than physiological transcript levels. These predictions were verified experimentally by overexpressing nine different asRNAs in Mycoplasma pneumoniae. Our results suggest that most of the antisense transcripts found in bacteria are the consequence of transcriptional noise, arising at spurious promoters throughout the genome.
- dc.description.sponsorship This work was supported by the European Union Seventh Framework Programme (FP7/2007–2013), through the European Research Council (232913); Fundación Botín, the Spanish Ministry of Economy and Competitiveness (BIO2007-61762); National Plan of R + D + i; ISCIII—Subdirección General de Evaluación y Fomento de la Investigación (PI10/01702); European Regional Development Fund (to the Institució Catalana de Recerca i Estudis Avançats research professor L.S.); and Spanish Ministry of Economy and Competitiveness, “Centro de Excelencia Severo Ochoa 2013–2017” (SEV-2012-0208). A.L. received grant BFU2012-39816-C02-01 from the Spanish Ministry of Economy and Competitivity cofinanced by FEDER (Fondo Europeo de Desarrollo Regional) funds.
- dc.format.mimetype application/pdfca
- dc.identifier.citation Lloréns-Rico V, Cano J, Kamminga T, Gil R, Latorre A, Chen W-H, Bork P, Glass JI, Serrano L, Lluch-Senar M. Bacterial antisense RNAs are mainly the product of transcriptional noise. Science Advances. 2016;2(3):e150363. DOI: 10.1126/sciadv.1501363
- dc.identifier.doi http://dx.doi.org/10.1126/sciadv.1501363
- dc.identifier.issn 2375-2548
- dc.identifier.uri http://hdl.handle.net/10230/30911
- dc.language.iso eng
- dc.publisher American Association for the Advancement of Science (AAAS)ca
- dc.relation.ispartof Science Advances. 2016;2(3):e150363
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/232913
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SEV2012-0208
- dc.rights Copyright © 2016, The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. DOI: 10.1126/sciadv.1501363
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri https://creativecommons.org/licenses/by-nc/4.0/
- dc.subject.keyword RNA
- dc.subject.keyword Bacterial antisense RNAs
- dc.title Bacterial antisense RNAs are mainly the product of transcriptional noiseca
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion