Characterization of human FDCs reveals regulation of T cells and antigen presentation to B cells

Mostra el registre complet Registre parcial de l'ítem

  • dc.contributor.author Heesters, Balthasar A.
  • dc.contributor.author van Megesen, Kyah
  • dc.contributor.author Tomris, Ilhan
  • dc.contributor.author de Vries, Robert P.
  • dc.contributor.author Magri, Giuliana, 1978-
  • dc.contributor.author Spits, Hergen
  • dc.date.accessioned 2022-06-22T06:55:53Z
  • dc.date.available 2022-06-22T06:55:53Z
  • dc.date.issued 2021
  • dc.description.abstract Stromal-derived follicular dendritic cells (FDCs) are essential for germinal centers (GCs), the site where B cells maturate their antibodies. FDCs present native antigen to B cells and maintain a CXCL13 gradient to form the B cell follicle. Yet despite their essential role, the transcriptome of human FDCs remains undefined. Using single-cell RNA sequencing and microarray, we provided the transcriptome of these enigmatic cells as a comprehensive resource. Key genes were validated by flow cytometry and microscopy. Surprisingly, marginal reticular cells (MRCs) rather than FDCs expressed B cell activating factor (BAFF). Furthermore, we found that human FDCs expressed TLR4 and can alter antigen availability in response to pathogen-associated molecular patterns (PAMPs). High expression of PD-L1 and PD-L2 on FDCs activated PD1 on T cells. In addition, we found expression of genes related to T cell regulation, such as HLA-DRA, CD40, and others. These data suggest intimate contact between human FDCs and T cells.
  • dc.description.sponsorship This work is supported by an ERC Starting Grant from the European Commission (802780 to R.P. de Vries) and a Dutch Research Council (NWO) ZonMw Veni fellowship (91618032 to B.A. Heesters).
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Heesters BA, van Megesen K, Tomris I, de Vries RP, Magri G, Spits H. Characterization of human FDCs reveals regulation of T cells and antigen presentation to B cells. J Exp Med. 2021 Oct 4; 218(10): e20210790. DOI: 10.1084/jem.20210790.
  • dc.identifier.doi http://dx.doi.org/10.1084/jem.20210790
  • dc.identifier.issn 0022-1007
  • dc.identifier.uri http://hdl.handle.net/10230/53560
  • dc.language.iso eng
  • dc.publisher Rockefeller University Press
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/802780
  • dc.rights © 2021 Heesters et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at http://creativecommons.org/licenses/by/4.0/).
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Cèl·lules dendrítiques
  • dc.subject.keyword Cèl·lules T
  • dc.subject.keyword Cèl·lules B
  • dc.title Characterization of human FDCs reveals regulation of T cells and antigen presentation to B cells
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

Col·leccions

Articles (Hospital del Mar Research Institute)
Documents OpenAIRE (Open Access Infrastructure for Research in Europe)