International external quality assurance of JAK2 V617F quantification

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• dc.contributor.author Asp, Julia
• dc.contributor.author Bellosillo Paricio, Beatriz
• dc.contributor.author Pallisgaard, Niels
• dc.date.accessioned 2019-06-28T07:36:13Z
• dc.date.available 2019-06-28T07:36:13Z
• dc.date.issued 2019
• dc.description.abstract External quality assurance (EQA) programs are vital to ensure high quality and standardized results in molecular diagnostics. It is important that EQA for quantitative analysis takes into account the variation in methodology. Results cannot be expected to be more accurate than limits of the technology used, and it is essential to recognize factors causing substantial outlier results. The present study aimed to identify parameters of specific importance for JAK2 V617F quantification by quantitative PCR, using different starting materials, assays, and technical platforms. Sixteen samples were issued to participating laboratories in two EQA rounds. In the first round, 19 laboratories from 11 European countries analyzing JAK2 V617F as part of their routine diagnostics returned results from in-house assays. In the second round, 25 laboratories from 17 countries participated. Despite variations in starting material, assay set-up and instrumentation the laboratories were generally well aligned in the EQA program. However, EQA based on a single technology appears to be a valuable tool to achieve standardization of the quantification of JAK2 V617F allelic burden.
• dc.format.mimetype application/pdf
• dc.identifier.citation Asp J, Skov V, Bellosillo B, Kristensen T, Lippert E, Dicker F. et al. International external quality assurance of JAK2 V617F quantification. Ann Hematol. 2019 May;98(5):1111-1118. DOI: 10.1007/s00277-018-3570-8
• dc.identifier.doi http://dx.doi.org/10.1007/s00277-018-3570-8
• dc.identifier.issn 0939-5555
• dc.identifier.uri http://hdl.handle.net/10230/41888
• dc.language.iso eng
• dc.publisher SpringerOpen
• dc.rights Copyright © The Author(s) 2018. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword External quality assurance
• dc.subject.keyword JAK2 V617F
• dc.subject.keyword Myeloproliferative neoplasms
• dc.subject.keyword Quantitative PCR
• dc.title International external quality assurance of JAK2 V617F quantification
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion