Olfactory neuroepithelium cells from cannabis users display alterations to the cytoskeleton and to markers of adhesion, proliferation and apoptosis
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Delgado-Sequera, Alejandra
- dc.contributor.author Hidalgo-Figueroa, María
- dc.contributor.author Barrera Conde, Marta
- dc.contributor.author Duran-Ruiz, M. Carmen
- dc.contributor.author Castro, Carmen
- dc.contributor.author Fernández Avilés, Cristina
- dc.contributor.author Torre Fornell, Rafael de la
- dc.contributor.author Sánchez-Gomar, Ismael
- dc.contributor.author Pérez, Victor
- dc.contributor.author Geribaldi-Doldán, Noelia
- dc.contributor.author Robledo, Patricia, 1958-
- dc.contributor.author Berrocoso, Esther
- dc.date.accessioned 2022-02-24T08:43:47Z
- dc.date.issued 2021
- dc.description.abstract Cannabis is the third most commonly used psychoactive substance of abuse, yet it also receives considerable attention as a potential therapeutic drug. Therefore, it is essential to fully understand the actions of cannabis in the human brain. The olfactory neuroepithelium (ON) is a peripheral nervous tissue that represents an interesting surrogate model to study the effects of drugs in the brain, since it is closely related to the central nervous system, and sensory olfactory neurons are continually regenerated from populations of stem/progenitor cells that undergo neurogenesis throughout life. In this study, we used ON cells from chronic cannabis users and healthy control subjects to assess alterations in relevant cellular processes, and to identify changes in functional proteomic pathways due to cannabis consumption. The ON cells from cannabis users exhibited alterations in the expression of proteins that were related to the cytoskeleton, cell proliferation and cell death, as well as, changes in proteins implicated in cancer, gastrointestinal and neurodevelopmental pathologies. Subsequent studies showed cannabis provoked an increase in cell size and morphological alterations evident through β-Tubulin III staining, as well as, enhanced beta-actin expression and a decrease in the ability of ON cells to undergo cell attachment, suggesting abnormalities of the cytoskeleton and cell adhesion system. Furthermore, these cells proliferated more and underwent less cell death. Our results indicate that cannabis may alter key processes of the developing brain, some of which are similar to those reported in mental disorders like DiGeorge syndrome, schizophrenia and bipolar disorder.
- dc.format.mimetype application/pdf
- dc.identifier.citation Delgado-Sequera A, Hidalgo-Figueroa M, Barrera-Conde M, Duran-Ruiz MC, Castro C, Fernández-Avilés C, de la Torre R, Sánchez-Gomar I, Pérez V, Geribaldi-Doldán N, Robledo P, Berrocoso E. Olfactory neuroepithelium cells from cannabis users display alterations to the cytoskeleton and to markers of adhesion, proliferation and apoptosis. Mol Neurobiol. 2021; 58(4):1695-710. DOI: 10.1007/s12035-020-02205-9
- dc.identifier.doi http://dx.doi.org/10.1007/s12035-020-02205-9
- dc.identifier.issn 0893-7648
- dc.identifier.uri http://hdl.handle.net/10230/52559
- dc.language.iso eng
- dc.publisher Springer
- dc.rights © Springer The final publication is available at Springer via http://dx.doi.org/10.1007/s12035-020-02205-9
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword Adhesion
- dc.subject.keyword Apoptosis
- dc.subject.keyword Cannabis
- dc.subject.keyword Cytoskeleton
- dc.subject.keyword Human olfactory Neuroepithelium
- dc.subject.keyword Proteomic
- dc.title Olfactory neuroepithelium cells from cannabis users display alterations to the cytoskeleton and to markers of adhesion, proliferation and apoptosis
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/acceptedVersion