Novel and optimized strategies for inducing fibrosis in vivo: focus on Duchenne Muscular Dystrophy

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• dc.contributor.author Pessina, Patriziaca
• dc.contributor.author Cabrera, Danielca
• dc.contributor.author Morales, María Gabrielaca
• dc.contributor.author Riquelme, Cecilia Aca
• dc.contributor.author Gutiérrez, Jaimeca
• dc.contributor.author Serrano, Antonio L.ca
• dc.contributor.author Brandan, Enriqueca
• dc.contributor.author Muñoz Cánoves, Pura, 1962-ca
• dc.date.accessioned 2015-03-24T12:07:57Z
• dc.date.available 2015-03-24T12:07:57Z
• dc.date.issued 2014ca
• dc.description.abstract Background: Fibrosis, an excessive collagen accumulation, results in scar formation, impairing function of vital organs and tissues. Fibrosis is a hallmark of muscular dystrophies, including the lethal Duchenne muscular dystrophy (DMD), which remains incurable. Substitution of muscle by fibrotic tissue also complicates gene/cell therapies for DMD. Yet, no optimal models to study muscle fibrosis are available. In the widely used mdx mouse model for DMD, extensive fibrosis develops in the diaphragm only at advanced adulthood, and at about two years of age in the ‘easy-to-access’ limb muscles, thus precluding fibrosis research and the testing of novel therapies.Methods: We developed distinct experimental strategies, ranging from chronic exercise to increasing muscle damage on limb muscles of young mdx mice, by myotoxin injection, surgically induced trauma (laceration or denervation) or intramuscular delivery of profibrotic growth factors (such as TGFβ). We also extended these approaches to muscle of normal non-dystrophic mice. Results: These strategies resulted in advanced and enhanced muscle fibrosis in young mdx mice, which persisted over time, and correlated with reduced muscle force, thus mimicking the severe DMD phenotype. Furthermore, increased fibrosis was also obtained by combining these procedures in muscles of normal mice, mirroring aberrant repair after severe trauma. Conclusions: We have developed new and improved experimental strategies to accelerate and enhance muscle fibrosis in vivo. These strategies will allow rapidly assessing fibrosis in the easily accessible limb muscles of young mdx mice, without necessarily having to use old animals. The extension of these fibrogenic regimes to the muscle of non-dystrophic wild-type mice will allow fibrosis assessment in a wide array of pre-existing transgenic mouse lines, which in turn will facilitate understanding the mechanisms of fibrogenesis. These strategies should improve our ability to combat fibrosis-driven dystrophy progression and aberrant regeneration.en
• dc.description.sponsorship The authors acknowledge funding from MINECO-Spain (SAF2012-38547, FIS-PS09/01267, FIS-PI13/02512, PLE2009-0124), AFM, E-Rare, Fundació-MaratóTV3, Duchenne PP-NL, EU-FP7 (Myoage, Optistem and Endostem), MDA, CARE PFB12/2007 and FONDECYT 1110426en
• dc.format.mimetype application/pdfca
• dc.identifier.citation Pessina P, Cabrera D, Morales MG, Riquelme CA, Gutiérrez J, Serrano AL et al. Novel and optimized strategies for inducing fibrosis in vivo: focus on Duchenne Muscular Dystrophy. Skeletal Muscle. 2014; 4: 7. DOI 10.1186/2044-5040-4-7ca
• dc.identifier.doi http://dx.doi.org/10.1186/2044-5040-4-7
• dc.identifier.issn 2044-5040ca
• dc.identifier.uri http://hdl.handle.net/10230/23265
• dc.language.iso engca
• dc.publisher BioMed Centralca
• dc.relation.ispartof Skeletal Muscle. 2014; 4: 7
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/223576ca
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2012-38547
• dc.rights © 2014 Pessina et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/2.0
• dc.subject.other Distròfia muscularca
• dc.subject.other Músculs -- Regeneracióca
• dc.title Novel and optimized strategies for inducing fibrosis in vivo: focus on Duchenne Muscular Dystrophyen
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca