ccSOL omics: a webserver for solubility prediction of endogenous and heterologous expression in Escherichia coli
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- dc.contributor.author Agostini, Federico, 1985-ca
- dc.contributor.author Cirillo, Davideca
- dc.contributor.author Livi, Carmen Mariaca
- dc.contributor.author Ponti, Riccardo dellica
- dc.contributor.author Tartaglia, Gian Gaetanoca
- dc.date.accessioned 2015-10-29T08:26:19Z
- dc.date.available 2015-10-29T08:26:19Z
- dc.date.issued 2014ca
- dc.description.abstract SUMMARY: Here we introduce ccSOL omics, a webserver for large-scale calculations of protein solubility. Our method allows (i) proteome-wide predictions; (ii) identification of soluble fragments within each sequences; (iii) exhaustive single-point mutation analysis. RESULTS: Using coil/disorder, hydrophobicity, hydrophilicity, β-sheet and α-helix propensities, we built a predictor of protein solubility. Our approach shows an accuracy of 79% on the training set (36 990 Target Track entries). Validation on three independent sets indicates that ccSOL omics discriminates soluble and insoluble proteins with an accuracy of 74% on 31 760 proteins sharing <30% sequence similarity. AVAILABILITY AND IMPLEMENTATION: ccSOL omics can be freely accessed on the web at http://s.tartaglialab.com/page/ccsol_group. Documentation and tutorial are available at http://s.tartaglialab.com/static_files/shared/tutorial_ccsol_omics.html. CONTACT: gian.tartaglia@crg.es/nSUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
- dc.description.sponsorship The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007–2013), through the European Research Council,under grant agreement RIBOMYLOME 309545, and from the Spanish Ministry of Economy and Competitiveness (SAF2011-26211). We also acknowledge support from the Spanish Ministry of Economy and Competitiveness, ‘Centro de Excelencia Severo Ochoa 2013–2017’ (SEV-2012-0208)
- dc.format.mimetype application/pdfca
- dc.identifier.citation Agostini F, Cirillo D, Livi CM, Delli Ponti R, Tartaglia GG. ccSOL omics: a/nwebserver for solubility prediction of endogenous and heterologous expression in Escherichia coli. Bioinformatics. 2014 Oct 15;30(20):2975-7. DOI: 10.1093/bioinformatics/btu420ca
- dc.identifier.doi http://dx.doi.org/10.1093/bioinformatics/btu420
- dc.identifier.issn 1367-4803ca
- dc.identifier.uri http://hdl.handle.net/10230/24951
- dc.language.iso engca
- dc.publisher Oxford University Pressca
- dc.relation.ispartof Bioinformatics. 2014 Oct 15;30(20):2975-7
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/309545
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2011-26211
- dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SEV-2012-0208
- dc.rights © 2014 Federico Agostini et al. Published by Oxford University Press. This is an Open Access article distributed under the terms of a Creative Commons Attribution Licenseca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri http://creativecommons.org/licenses/by/3.0/
- dc.subject.other Escheríchia coli
- dc.subject.other Proteïnes
- dc.subject.other Expressió gènica
- dc.subject.other Proteòmica
- dc.title ccSOL omics: a webserver for solubility prediction of endogenous and heterologous expression in Escherichia colica
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca