Simplified method for the measurement of plasma alkylresorcinols: Biomarkers of whole-grain intake

Mostra el registre complet Registre parcial de l'ítem

  • dc.contributor.author Rodríguez-Morató, Jose, 1987-
  • dc.contributor.author Jayawardene, Sarah
  • dc.contributor.author Huang, Neil K.
  • dc.contributor.author Dolnikowski, Gregory G.
  • dc.contributor.author Galluccio, Jean M.
  • dc.contributor.author Lichtenstein, Alice H.
  • dc.contributor.author Matthan, Nirupa R.
  • dc.date.accessioned 2020-12-11T07:27:02Z
  • dc.date.issued 2020
  • dc.description.abstract Rationale: Consumption of whole grains is negatively associated with cardiovascular disease (CVD) risk but quantification of whole-grain intake is challenging. Alkylresorcinols (ARs) are biomarkers of whole-grain intake. Current methods for AR quantification involve a time-consuming multi-step separation process that hampers applicability in large-scale studies. Methods: We developed a streamlined method to quantify ARs in human plasma based on protein precipitation and direct injection into an ultra-high-performance liquid chromatograph coupled to a quadrupole time-of-flight mass spectrometer operating in atmospheric pressure chemical ionization negative ion mode. Results: Separation of five major ARs was achieved, with linearity in the 5 to 550 nmol/L range and a lower limit of detection (LOD) of 0.5 nmol/L and quantification (LOQ) of 5 nmol/L. The within-run and between-run precision and accuracy were below 15%, and recoveries above 90%. Once validated, the method was applied to measure concentrations of plasma ARs in subjects who participated in a randomized, crossover trial evaluating the effect of carbohydrate type on CVD risk factors. The unrefined carbohydrate diet with the highest fiber content resulted in the highest plasma AR concentration (93 ± 78 nmol/L), and was significantly different (p <0.01) from lower fiber diets (18 ± 26 nmol/L and 19 ± 26 nmol/L, simple and unrefined carbohydrate, respectively). Conclusions: This method offers a simplified approach to measure concentrations of plasma ARs as an objective biomarker of whole-grain intake that can be applied to large-scale cohort studies.
  • dc.description.sponsorship This work was supported by the U.S. Department of Agriculture (agreement no. 58-1950-4-401), and pilot funds from the Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University. Any opinions, findings, conclusions or recommendations expressed in this publication are those of authors, and do not necessarily reflect the view of the U. S. Department of Agriculture. JR-M acknowledges funding from EU Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 712949 (TECNIOspring PLUS) and from ACCIO.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Rodríguez-Morató J, Jayawardene S, Huang NK, Dolnikowski GG, Galluccio J, Lichtenstein AH, Matthan NR. Simplified method for the measurement of plasma alkylresorcinols: Biomarkers of whole-grain intake. Rapid Commun Mass Spectrom. 2020; 34(14):e8805. DOI: 10.1002/rcm.8805
  • dc.identifier.doi http://dx.doi.org/10.1002/rcm.8805
  • dc.identifier.issn 0951-4198
  • dc.identifier.uri http://hdl.handle.net/10230/45978
  • dc.language.iso eng
  • dc.publisher Wiley
  • dc.relation.ispartof Rapid Commun Mass Spectrom. 2020; 34(14):e8805
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/712949
  • dc.rights This is the peer reviewed version of the following article: Rodríguez-Morató J, Jayawardene S, Huang NK, Dolnikowski GG, Galluccio J, Lichtenstein AH, Matthan NR. Simplified method for the measurement of plasma alkylresorcinols: Biomarkers of whole-grain intake. Rapid Commun Mass Spectrom. 2020; 34(14):e8805. DOI: 10.1002/rcm.8805, which has been published in final form at http://dx.doi.org/10.1002/rcm.8805. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.subject.keyword Alkylresorcinols
  • dc.subject.keyword Biomarkers
  • dc.subject.keyword Liquid chromatography
  • dc.subject.keyword Randomized clinical trial
  • dc.subject.keyword Whole-grains
  • dc.title Simplified method for the measurement of plasma alkylresorcinols: Biomarkers of whole-grain intake
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/acceptedVersion

Col·leccions

Articles (Departament de Medicina i Ciències de la Vida)
Documents OpenAIRE (Open Access Infrastructure for Research in Europe)