FLCCR is a fluorescent reporter system that quantifies the duration of different cell cycle phases at the single-cell level in fission yeast

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• dc.contributor.author Murciano-Julià, Guillem
• dc.contributor.author Francos Cárdenas, Marina
• dc.contributor.author Salat Canela, Clàudia, 1989-
• dc.contributor.author Hidalgo Hernando, Elena
• dc.date.accessioned 2025-02-21T07:16:31Z
• dc.date.available 2025-02-21T07:16:31Z
• dc.date.issued 2025
• dc.description.abstract Fission yeast is an excellent model system that has been widely used to study the mechanism that control cell cycle progression. However, there is a lack of tools that allow to measure with high precision the duration of the different phases of the cell cycle in individual cells. To circumvent this problem, we have developed a fluorescent reporter that allows the quantification of the different phases of the cell cycle at the single-cell level in most genetic backgrounds. To prove the accuracy of this fluorescent reporter, we have tested the reporter in strains known to have a delay in the G1/S or G2/M transitions, confirming the strength and versatility of the system. An advantage of this reporter is that it eliminates the need for culture synchronization, avoiding stressing the cells. Using this reporter, we show that unperturbed cells lacking Sty1 have a standard cell cycle length and distribution and that the extended length of these cells is due to their increased cell growth rate but not to alterations in their cell cycle progression.
• dc.description.sponsorship This work was supported by grant PID2022-136449NB-I00 funded by MICIU/AEI/10.13039/501100011033 and ERDF/EU to J.A. and by Unidad de Excelencia Maria de Maeztu (CEX2018-000792-M) to J.A. and E.H. E.H. is a recipient of an ICREA Academia Award (Generalitat de Catalunya). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
• dc.format.mimetype application/pdf
• dc.identifier.citation Murciano-Julià G, Francos-Cárdenas M, Salat-Canela C, Hidalgo E, Ayté J. FLCCR is a fluorescent reporter system that quantifies the duration of different cell cycle phases at the single-cell level in fission yeast. PLoS Biol. 2025 Jan 7;23(1):e3002969. DOI: 10.1371/journal.pbio.3002969
• dc.identifier.doi http://dx.doi.org/10.1371/journal.pbio.3002969
• dc.identifier.issn 1544-9173
• dc.identifier.uri http://hdl.handle.net/10230/69674
• dc.language.iso eng
• dc.publisher Public Library of Science (PLoS)
• dc.relation.ispartof PLoS Biol. 2025 Jan 7;23(1):e3002969
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2022-136449NB-I00
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M
• dc.rights © 2025 Murciano-Julià et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Cell cycle and cell division
• dc.subject.keyword Nuclear membrane
• dc.subject.keyword Mitosis
• dc.subject.keyword Synthesis phase
• dc.subject.keyword G2 phase
• dc.subject.keyword Schizosaccharomyces pombe
• dc.subject.keyword G1 phase
• dc.subject.keyword Plasmid construction
• dc.title FLCCR is a fluorescent reporter system that quantifies the duration of different cell cycle phases at the single-cell level in fission yeast
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion